Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients
| Title: | Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients |
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| Authors: | Javad Mahmoudi, Babak Sabermarouf, Behzad Baradaran, Leila Sadat-Hatamnezhad, Siamak Sandoghchian Shotorbani |
| Source: | Iranian Journal of Basic Medical Sciences, Vol 20, Iss 2, Pp 209-215 (2017) |
| Publisher Information: | Mashhad University of Medical Sciences, 2017. |
| Publication Year: | 2017 |
| Collection: | LCC:Medicine |
| Subject Terms: | HMGB1, Pulpitis, TLR2, TLR4, Medicine |
| More Details: | Objective(s): High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes. Materials and Methods: Having prepared dental pulp tissues of pulpitis and healthy controls, macrophage were isolated and cultured. Macrophages were thereafter stimulated by HMGB1 time course. RT-QPCR, flowcytometer, immunofluorescence, Western blotting, and ELISA techniques were used in the present research. Results: Our results showed that the expression of TLR2 and TLR4 on macrophages stimulated with HMGB1 increased in pulpitis compared with controls (macrophages without HMGB1 stimulation) with a statistical significance (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2008-3866 2008-3874 |
| Relation: | http://ijbms.mums.ac.ir/article_8250_3b98ce9154b9aecf3ce2d86fb6b01e14.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2017.8250 |
| Access URL: | https://doaj.org/article/ebeb52d809bf4491a16d9f7163d80764 |
| Accession Number: | edsdoj.beb52d809bf4491a16d9f7163d80764 |
| Database: | Directory of Open Access Journals |
| ISSN: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2017.8250 |
| Published in: | Iranian Journal of Basic Medical Sciences |
| Language: | English |