Bibliographic Details
| Title: |
A Combined Proteomic and Metabolomic Strategy for Allergens Characterization in Natural and Fermented Brassica napus Bee Pollen |
| Authors: |
Shuting Yin, Yuxiao Tao, Yusuo Jiang, Lifeng Meng, Liuwei Zhao, Xiaofeng Xue, Qiangqiang Li, Liming Wu |
| Source: |
Frontiers in Nutrition, Vol 9 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Nutrition. Foods and food supply |
| Subject Terms: |
Brassica napus bee pollen, allergens, degradation and catabolism, fermentation, Saccharomyces cerevisiae, Nutrition. Foods and food supply, TX341-641 |
| More Details: |
Bee pollen is consumed for its nutritional and pharmacological benefits, but it also contains hazardous allergens which have not been identified. Here, we identified two potential allergens, glutaredoxin and oleosin-B2, in Brassica napus bee pollen using mass spectrometry-based proteomics analyses, and used bioinformatics to predict their antigenic epitopes. Comparison of fermented (by Saccharomyces cerevisiae) and unfermented bee pollen samples indicated that glutaredoxin and oleosin-B2 contents were significantly decreased following fermentation, while the contents of their major constituent oligopeptides and amino acids were significantly increased based on metabolomics analyses. Immunoblot analysis indicated that the IgE-binding affinity with extracted bee pollen proteins was also significantly decreased after fermentation, suggesting a reduction in the allergenicity of fermented bee pollen. Furthermore, fermentation apparently promoted the biosynthesis of L-valine, L-isoleucine, L-tryptophan, and L-phenylalanine, as well as their precursors or intermediates. Thus, fermentation could potentially alleviate allergenicity, while also positively affecting nutritional properties of B. napus bee pollen. Our findings might provide a scientific foundation for improving the safety of bee pollen products to facilitate its wider application. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-861X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fnut.2022.822033/full; https://doaj.org/toc/2296-861X |
| DOI: |
10.3389/fnut.2022.822033 |
| Access URL: |
https://doaj.org/article/be3bdc78963e41dc80d7a5bd6c9d07f9 |
| Accession Number: |
edsdoj.be3bdc78963e41dc80d7a5bd6c9d07f9 |
| Database: |
Directory of Open Access Journals |
