Academic Journal
View in EDS

Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology

Bibliographic Details
Title: Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology
Authors: L. Pedrosa, I. K. Araujo, M. Cuatrecasas, G. Soy, S. López, J. Maurel, C. Sánchez-Montes, C. Montironi, T. Saurí, O. Sendino, F. M. Pérez, F. Ausania, G. Fernández-Esparrach, F. M. Espósito, E. C. Vaquero, A. Ginès
Source: Frontiers in Molecular Biosciences, Vol 10 (2023)
Publisher Information: Frontiers Media S.A., 2023.
Publication Year: 2023
Collection: LCC:Biology (General)
Subject Terms: pancreatic adenocarcinoma, EUS-FNA, NanoString technology, tumour stroma, transcriptomic analysis, Biology (General), QH301-705.5
More Details: Background: Integration of transcriptomic testing into EUS-FNA samples is a growing need for precision oncology in pancreatic ductal adenocarcinoma (PDAC). The NanoString platform is suitable for transcriptome profiling in low yield RNA samples.Methods: Inclusion of patients that underwent EUS-FNA cytological diagnosis of pancreatic ductal adenocarcinoma using 19G and/or 22G needles and subsequent surgical resection. Formalin-fixed, paraffin-embedded (FFPE) cytological and surgical samples underwent RNA extraction and transcriptomic analysis using a custom 52-gene NanoString panel of stromal PDAC features. Cell type abundance was quantified in FFPE specimens and correlated.Results: 18 PDAC patients were included. Mean EUS-FNA passes was 2 + 0.7. All FFPE passed the RNA quality control for genomic analysis. Hierarchical clustering on the global gene expression data showed that genes were differentially expressed between EUS and surgical samples. A more enriched cancer-associated fibroblasts and epithelial-mesenchymal transition transcriptomic profile was observed across surgical specimens whereas immunological biomarkers were more represented in EUS-FNA samples. Cytological examination confirmed a scanty representation of CAF and more immunological cell abundance in cytological samples in comparison to surgical specimens.Conclusion: Targeted transcriptomic NanoString profiling of PDAC samples obtained by EUS-FNA is a feasible approach for pre-surgical molecular analysis although stromal CAF/EMT mRNA biomarkers are underrepresented.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2296-889X
Relation: https://www.frontiersin.org/articles/10.3389/fmolb.2023.1161893/full; https://doaj.org/toc/2296-889X
DOI: 10.3389/fmolb.2023.1161893
Access URL: https://doaj.org/article/bd781e11ebac4838b6d93f1bb894f47a
Accession Number: edsdoj.bd781e11ebac4838b6d93f1bb894f47a
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=2296889X&ISBN=&volume=10&issue=&date=20230501&spage=&pages=&title=Frontiers in Molecular Biosciences&atitle=Targeted%20transcriptomic%20analysis%20of%20pancreatic%20adenocarcinoma%20in%20EUS-FNA%20samples%20by%20NanoString%20technology&aulast=L.%20Pedrosa&id=DOI:10.3389/fmolb.2023.1161893
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/bd781e11ebac4838b6d93f1bb894f47a
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.bd781e11ebac4838b6d93f1bb894f47a
RelevancyScore: 967
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 967.328796386719
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22L%2E+Pedrosa%22">L. Pedrosa</searchLink><br /><searchLink fieldCode="AR" term="%22I%2E+K%2E+Araujo%22">I. K. Araujo</searchLink><br /><searchLink fieldCode="AR" term="%22M%2E+Cuatrecasas%22">M. Cuatrecasas</searchLink><br /><searchLink fieldCode="AR" term="%22G%2E+Soy%22">G. Soy</searchLink><br /><searchLink fieldCode="AR" term="%22S%2E+López%22">S. López</searchLink><br /><searchLink fieldCode="AR" term="%22J%2E+Maurel%22">J. Maurel</searchLink><br /><searchLink fieldCode="AR" term="%22C%2E+Sánchez-Montes%22">C. Sánchez-Montes</searchLink><br /><searchLink fieldCode="AR" term="%22C%2E+Montironi%22">C. Montironi</searchLink><br /><searchLink fieldCode="AR" term="%22T%2E+Saurí%22">T. Saurí</searchLink><br /><searchLink fieldCode="AR" term="%22O%2E+Sendino%22">O. Sendino</searchLink><br /><searchLink fieldCode="AR" term="%22F%2E+M%2E+Pérez%22">F. M. Pérez</searchLink><br /><searchLink fieldCode="AR" term="%22F%2E+Ausania%22">F. Ausania</searchLink><br /><searchLink fieldCode="AR" term="%22G%2E+Fernández-Esparrach%22">G. Fernández-Esparrach</searchLink><br /><searchLink fieldCode="AR" term="%22F%2E+M%2E+Espósito%22">F. M. Espósito</searchLink><br /><searchLink fieldCode="AR" term="%22E%2E+C%2E+Vaquero%22">E. C. Vaquero</searchLink><br /><searchLink fieldCode="AR" term="%22A%2E+Ginès%22">A. Ginès</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Frontiers in Molecular Biosciences, Vol 10 (2023)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Frontiers Media S.A., 2023.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2023
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Biology (General)
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22pancreatic+adenocarcinoma%22">pancreatic adenocarcinoma</searchLink><br /><searchLink fieldCode="DE" term="%22EUS-FNA%22">EUS-FNA</searchLink><br /><searchLink fieldCode="DE" term="%22NanoString+technology%22">NanoString technology</searchLink><br /><searchLink fieldCode="DE" term="%22tumour+stroma%22">tumour stroma</searchLink><br /><searchLink fieldCode="DE" term="%22transcriptomic+analysis%22">transcriptomic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+%28General%29%22">Biology (General)</searchLink><br /><searchLink fieldCode="DE" term="%22QH301-705%2E5%22">QH301-705.5</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Background: Integration of transcriptomic testing into EUS-FNA samples is a growing need for precision oncology in pancreatic ductal adenocarcinoma (PDAC). The NanoString platform is suitable for transcriptome profiling in low yield RNA samples.Methods: Inclusion of patients that underwent EUS-FNA cytological diagnosis of pancreatic ductal adenocarcinoma using 19G and/or 22G needles and subsequent surgical resection. Formalin-fixed, paraffin-embedded (FFPE) cytological and surgical samples underwent RNA extraction and transcriptomic analysis using a custom 52-gene NanoString panel of stromal PDAC features. Cell type abundance was quantified in FFPE specimens and correlated.Results: 18 PDAC patients were included. Mean EUS-FNA passes was 2 + 0.7. All FFPE passed the RNA quality control for genomic analysis. Hierarchical clustering on the global gene expression data showed that genes were differentially expressed between EUS and surgical samples. A more enriched cancer-associated fibroblasts and epithelial-mesenchymal transition transcriptomic profile was observed across surgical specimens whereas immunological biomarkers were more represented in EUS-FNA samples. Cytological examination confirmed a scanty representation of CAF and more immunological cell abundance in cytological samples in comparison to surgical specimens.Conclusion: Targeted transcriptomic NanoString profiling of PDAC samples obtained by EUS-FNA is a feasible approach for pre-surgical molecular analysis although stromal CAF/EMT mRNA biomarkers are underrepresented.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 2296-889X
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://www.frontiersin.org/articles/10.3389/fmolb.2023.1161893/full; https://doaj.org/toc/2296-889X
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3389/fmolb.2023.1161893
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/bd781e11ebac4838b6d93f1bb894f47a" linkWindow="_blank">https://doaj.org/article/bd781e11ebac4838b6d93f1bb894f47a</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.bd781e11ebac4838b6d93f1bb894f47a
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.bd781e11ebac4838b6d93f1bb894f47a
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3389/fmolb.2023.1161893
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: pancreatic adenocarcinoma
        Type: general
      – SubjectFull: EUS-FNA
        Type: general
      – SubjectFull: NanoString technology
        Type: general
      – SubjectFull: tumour stroma
        Type: general
      – SubjectFull: transcriptomic analysis
        Type: general
      – SubjectFull: Biology (General)
        Type: general
      – SubjectFull: QH301-705.5
        Type: general
    Titles:
      – TitleFull: Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: L. Pedrosa
      – PersonEntity:
          Name:
            NameFull: I. K. Araujo
      – PersonEntity:
          Name:
            NameFull: M. Cuatrecasas
      – PersonEntity:
          Name:
            NameFull: G. Soy
      – PersonEntity:
          Name:
            NameFull: S. López
      – PersonEntity:
          Name:
            NameFull: J. Maurel
      – PersonEntity:
          Name:
            NameFull: C. Sánchez-Montes
      – PersonEntity:
          Name:
            NameFull: C. Montironi
      – PersonEntity:
          Name:
            NameFull: T. Saurí
      – PersonEntity:
          Name:
            NameFull: O. Sendino
      – PersonEntity:
          Name:
            NameFull: F. M. Pérez
      – PersonEntity:
          Name:
            NameFull: F. Ausania
      – PersonEntity:
          Name:
            NameFull: G. Fernández-Esparrach
      – PersonEntity:
          Name:
            NameFull: F. M. Espósito
      – PersonEntity:
          Name:
            NameFull: E. C. Vaquero
      – PersonEntity:
          Name:
            NameFull: A. Ginès
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 05
              Type: published
              Y: 2023
          Identifiers:
            – Type: issn-print
              Value: 2296889X
          Numbering:
            – Type: volume
              Value: 10
          Titles:
            – TitleFull: Frontiers in Molecular Biosciences
              Type: main
ResultId 1