Bibliographic Details
| Title: |
Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology |
| Authors: |
L. Pedrosa, I. K. Araujo, M. Cuatrecasas, G. Soy, S. López, J. Maurel, C. Sánchez-Montes, C. Montironi, T. Saurí, O. Sendino, F. M. Pérez, F. Ausania, G. Fernández-Esparrach, F. M. Espósito, E. C. Vaquero, A. Ginès |
| Source: |
Frontiers in Molecular Biosciences, Vol 10 (2023) |
| Publisher Information: |
Frontiers Media S.A., 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
pancreatic adenocarcinoma, EUS-FNA, NanoString technology, tumour stroma, transcriptomic analysis, Biology (General), QH301-705.5 |
| More Details: |
Background: Integration of transcriptomic testing into EUS-FNA samples is a growing need for precision oncology in pancreatic ductal adenocarcinoma (PDAC). The NanoString platform is suitable for transcriptome profiling in low yield RNA samples.Methods: Inclusion of patients that underwent EUS-FNA cytological diagnosis of pancreatic ductal adenocarcinoma using 19G and/or 22G needles and subsequent surgical resection. Formalin-fixed, paraffin-embedded (FFPE) cytological and surgical samples underwent RNA extraction and transcriptomic analysis using a custom 52-gene NanoString panel of stromal PDAC features. Cell type abundance was quantified in FFPE specimens and correlated.Results: 18 PDAC patients were included. Mean EUS-FNA passes was 2 + 0.7. All FFPE passed the RNA quality control for genomic analysis. Hierarchical clustering on the global gene expression data showed that genes were differentially expressed between EUS and surgical samples. A more enriched cancer-associated fibroblasts and epithelial-mesenchymal transition transcriptomic profile was observed across surgical specimens whereas immunological biomarkers were more represented in EUS-FNA samples. Cytological examination confirmed a scanty representation of CAF and more immunological cell abundance in cytological samples in comparison to surgical specimens.Conclusion: Targeted transcriptomic NanoString profiling of PDAC samples obtained by EUS-FNA is a feasible approach for pre-surgical molecular analysis although stromal CAF/EMT mRNA biomarkers are underrepresented. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-889X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmolb.2023.1161893/full; https://doaj.org/toc/2296-889X |
| DOI: |
10.3389/fmolb.2023.1161893 |
| Access URL: |
https://doaj.org/article/bd781e11ebac4838b6d93f1bb894f47a |
| Accession Number: |
edsdoj.bd781e11ebac4838b6d93f1bb894f47a |
| Database: |
Directory of Open Access Journals |
