Academic Journal
Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups
| Title: | Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups |
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| Authors: | Javier Escobar, Elena Cubells, Masahiro Enomoto, Guillermo Quintás, Julia Kuligowski, Cristina Martinez Fernández, Isabel Torres-Cuevas, Juan Sastre, Jaques Belik, Máximo Vento |
| Source: | Redox Biology, Vol 1, Iss 1, Pp 297-303 (2013) |
| Publisher Information: | Elsevier, 2013. |
| Publication Year: | 2013 |
| Collection: | LCC:Medicine (General) LCC:Biology (General) |
| Subject Terms: | Fetal-to-neonatal transition, Oxygen, Oxidative stress, Redox regulation, Glutathione, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| More Details: | Background: Fetal-to-neonatal transition is associated with oxidative stress. In preterm infants, immaturity of the antioxidant system favours supplemental oxygen-derived morbidity and mortality. Objectives: To assess if prolonging in utero-like oxygenation during the fetal-to-neonatal transition limits oxidative stress in the lung and brain, improving postnatal adaptation of mice pups. Material and methods: Inspiratory oxygen fraction (FiO2) in pregnant mice was reduced from 21% (room air) to 14% (hypoxia) 8–12 h prior to delivery and reset to 21% 6–8 h after birth. The control group was kept at 21% during the procedure. Reduced (GSH) and oxidized (GSSG) glutathione and its precursors [γ-glutamyl cysteine (γ-GC) and L-cysteine (CySH)] content and expression of several redox-sensitive genes were evaluated in newborn lung and brain tissue 1 (P1) and 7 (P7) days after birth. Results: As compared with control animals, the GSH/GSSG ratio was increased in the hypoxic group at P1 and P7 in the lung, and at P7 in the brain. In the hypoxic group a significant increase in the mRNA levels of NAD(P)H:quinone oxidoreductase 1 (noq1), Sulfiredoxin 1 (srnx1) and Glutathione Peroxidase 1 (gpx) was found in lung tissue at P1, as well as a significant increase in gpx in brain tissue at P7. Conclusions: Delaying the increase in tissue oxygenation to occur after birth reduces short-and-long-term oxidative stress in the lung. Similar yet more subtle effects were found in the brain. Apparently, the fetal-to-neonatal transition under hypoxic conditions appears to have protective qualities. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2213-2317 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213231713000402; https://doaj.org/toc/2213-2317 |
| DOI: | 10.1016/j.redox.2013.04.002 |
| Access URL: | https://doaj.org/article/bd47969de79b476a8a77c13daa5706d4 |
| Accession Number: | edsdoj.bd47969de79b476a8a77c13daa5706d4 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22132317 |
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| DOI: | 10.1016/j.redox.2013.04.002 |
| Published in: | Redox Biology |
| Language: | English |