| Title: |
Development of a Mild Viral Expression System for Gain-Of-Function Study of Phytoplasma Effector In Planta. |
| Authors: |
Sin-Fen Hu, Yu-Hsin Huang, Chan-Pin Lin, Li-Yu Daisy Liu, Syuan-Fei Hong, Chiao-Yin Yang, Hsiao-Feng Lo, Ting-Yu Tseng, Wei-Yao Chen, Shih-Shun Lin |
| Source: |
PLoS ONE, Vol 10, Iss 6, p e0130139 (2015) |
| Publisher Information: |
Public Library of Science (PLoS), 2015. |
| Publication Year: |
2015 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
PHYL1 and SAP54 are orthologs of pathogenic effectors of Aster yellow witches'-broom (AYWB) phytoplasma and Peanut witches'-broom (PnWB) phytoplasma, respectively. These effectors cause virescence and phyllody symptoms (hereafter leafy flower) in phytoplasma-infected plants. T0 lines of transgenic Arabidopsis expressing the PHYL1 or SAP54 genes (PHYL1 or SAP54 plants) show a leafy flower phenotype and result in seedless, suggesting that PHYL1 and SAP54 interfere with reproduction stage that restrict gain-of-function studies in the next generation of transgenic plants. Turnip mosaic virus (TuMV) mild strain (TuGK) has an Arg182Lys mutation in the helper-component proteinase (HC-ProR182K) that blocks suppression of the miRNA pathway and prevents symptom development in TuGK-infected plants. We exploited TuGK as a viral vector for gain-of-function studies of PHYL1 and SAP54 in Arabidopsis plants. TuGK-PHYL1- and TuGK-SAP54-infected Arabidopsis plants produced identical leafy flower phenotypes and similar gene expression profiles as PHYL1 and SAP54 plants. In addition, the leafy flower formation rate was enhanced in TuGK-PHYL1- or TuGK-SAP54-infected Arabidopsis plants that compared with the T0 lines of PHYL1 plants. These results provide more evidence and novel directions for further studying the mechanism of PHYL1/SAP54-mediated leafy flower development. In addition, the TuGK vector is a good alternative in transgenic plant approaches for rapid gene expression in gain-of-function studies. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
http://europepmc.org/articles/PMC4468105?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0130139 |
| Access URL: |
https://doaj.org/article/dcbccd31d20f472fb085f21c05081201 |
| Accession Number: |
edsdoj.bccd31d20f472fb085f21c05081201 |
| Database: |
Directory of Open Access Journals |
