Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
| Title: | Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo |
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| Authors: | Elvira Maria Mendes Nascimento, Flávia de Sousa Gehrke, Rosa Amélia Maldonado, Silvia Colombo, Luiz Jacintho da Silva, Teresinha Tizu Sato Schumaker |
| Source: | Memorias do Instituto Oswaldo Cruz, Vol 100, Iss 3, Pp 277-279 (2005) |
| Publisher Information: | Fundação Oswaldo Cruz (FIOCRUZ), 2005. |
| Publication Year: | 2005 |
| Collection: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| Subject Terms: | Brazilian spotted fever, Rickettsia rickettsii, ompB gene, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| More Details: | Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0074-0276 1678-8060 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300011; https://doaj.org/toc/0074-0276; https://doaj.org/toc/1678-8060 |
| DOI: | 10.1590/S0074-02762005000300011 |
| Access URL: | https://doaj.org/article/bc30b9ac6f2c4b02874cde88fbda58f3 |
| Accession Number: | edsdoj.bc30b9ac6f2c4b02874cde88fbda58f3 |
| Database: | Directory of Open Access Journals |
| ISSN: | 00740276 16788060 |
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| DOI: | 10.1590/S0074-02762005000300011 |
| Published in: | Memorias do Instituto Oswaldo Cruz |
| Language: | English |