Academic Journal
Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling
| Title: | Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling |
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| Authors: | Dingding Wang, Xinhao Zhang, Jianxun Guo, Weijia Liu, Yanchi Zhou, Renxian Wang |
| Source: | Biochemistry and Biophysics Reports, Vol 40, Iss , Pp 101864- (2024) |
| Publisher Information: | Elsevier, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Biology (General) LCC:Biochemistry |
| Subject Terms: | TGF-β signaling, EXOSC10, Kinome-wide CRISPR screen, Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| More Details: | The TGF-β signaling pathway is closely associated with human health and disease, and the systematic identification of factors involved in the TGF-β signaling pathway significantly contributes to the understanding and treatment of various diseases. Through kinome-wide CRISPR screen, we identified 13 candidate regulatory targets. Notably, the well-known hallmark genes TGFBR1 and TGFBR2 emerged as the top two candidate targets. OXSR1 and EXOSC10 were ranked third and fourth as positive candidate targets, respectively, with EXOSC10 being a novel discovery. Importantly, our findings revealed the down-regulation of OXSR1 and EXOSC10 using CRISPR knockout and RNAi technology effectively suppressed the TGF-β signaling pathway in HeLa and HaCaT cells, providing new insights of TGF-β signaling. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2405-5808 43694934 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405580824002280; https://doaj.org/toc/2405-5808 |
| DOI: | 10.1016/j.bbrep.2024.101864 |
| Access URL: | https://doaj.org/article/aecbb43694934f1ab4f7ac48b6dfb829 |
| Accession Number: | edsdoj.bb43694934f1ab4f7ac48b6dfb829 |
| Database: | Directory of Open Access Journals |
| ISSN: | 24055808 43694934 |
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| DOI: | 10.1016/j.bbrep.2024.101864 |
| Published in: | Biochemistry and Biophysics Reports |
| Language: | English |