Bibliographic Details
| Title: |
Generation of a KCNQ1 (c.1032 + 2 T > C) mutant human embryonic stem cell line via CRISPR base editing |
| Authors: |
Xiaodong Jiang, Chong Fu, Qiying Liu, Jiaqi Gao |
| Source: |
Stem Cell Research, Vol 77, Iss , Pp 103425- (2024) |
| Publisher Information: |
Elsevier, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
Biology (General), QH301-705.5 |
| More Details: |
The KCNQ1 gene encodes a voltage-gated potassium channel, which plays an important role in the repolarization of myocardial action potentials. Mutations in this gene often result in type 1 long QT syndrome (LQT1). Here, we generated a KCNQ1 (c.1032 + 2 T > C) mutant human embryonic stem cell line (WAe009-A-1D) based on the transient expression adenine base editing system that converts base A to G. The WAe009-A-1D cell maintains the morphology, pluripotency, and normal karyotype of the stem cells and is capable of differentiating into all three germ layers in vivo. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1873-5061 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1873506124001235; https://doaj.org/toc/1873-5061 |
| DOI: |
10.1016/j.scr.2024.103425 |
| Access URL: |
https://doaj.org/article/ecb976b0701c4e0bacdbb554e4f710ba |
| Accession Number: |
edsdoj.b976b0701c4e0bacdbb554e4f710ba |
| Database: |
Directory of Open Access Journals |
