Bibliographic Details
| Title: |
Production of a chimeric porcine reproductive and respiratory syndrome virus (PRRSV)-2 vaccine using a lab-scale packed-bed bioreactor CelCradle |
| Authors: |
Hwi-Yeon Choi, Jong-Chul Choi, Yeong-Lim Kang, So-Hyeun Ahn, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee |
| Source: |
BMC Veterinary Research, Vol 19, Iss 1, Pp 1-12 (2023) |
| Publisher Information: |
BMC, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Veterinary medicine |
| Subject Terms: |
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Vaccine production, CelCradle, Packed Bed, Bioreactor, Vaccine efficacy, Veterinary medicine, SF600-1100 |
| More Details: |
Abstract Background We developed a MARC-145 cell culture and porcine reproductive and respiratory syndrome (PRRS) vaccine production using a novel CelCradle bioreactor. CelCradle is a packed-bed bioreactor capable of both batch and perfusion culture, and the operating parameters are easy to optimize. Results In this study, CelCradle reached a maximum cell density of 8.94 × 105 cells/mL at 5 days post-seeding when seeded at 8.60 × 104 cells/mL (doubling time = 35.52 h). Inoculation of PRRS vaccine candidate, K418DM1.1, was performed at a multiplicity of infection (MOI) of 0.01 at 5 days post-seeding, which resulted in a high viral titer of 2.04 × 108 TCID50/mL and total viral load of 1.02 × 1011 TCID50/500 mL at 2 days post-infection (dpi). The multilayer cultivation system, BioFactory culture, yielded a higher doubling time (37.14 h) and lower viral titer (i.e., 8.15 × 107 TCID50/mL) compared to the CelCradle culture. Thus, the culture medium productivity of the CelCradle culture was 2-fold higher than that of the BioFactory culture. In the animal experiment, the CelCradle-produced vaccine induced high levels of neutralizing antibodies and effectively protected pigs against homologous challenge, as shown by the significantly lower levels of viremia at 1- and 7-days post-challenge (dpc) compared to the non-vaccinated pigs. Conclusions Overall, this study demonstrates that the CelCradle system is an economical platform for PRRS vaccine production. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1746-6148 |
| Relation: |
https://doaj.org/toc/1746-6148 |
| DOI: |
10.1186/s12917-023-03659-4 |
| Access URL: |
https://doaj.org/article/b9720a16908646f2a4ff21730b479269 |
| Accession Number: |
edsdoj.b9720a16908646f2a4ff21730b479269 |
| Database: |
Directory of Open Access Journals |
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