| Title: |
Metabolomic and proteomic profiling of a burn-hemorrhagic shock swine model reveals a metabolomic signature associated with fatal outcomes |
| Authors: |
Bin Wei, Jinguang Zheng, Jiake Chai, Jianxiang Huang, Hongjie Duan, Shaofang Han, Xiaolin Yang, Wenjia Zhang, Fangchao Hu, Yirui Qu, Xiangyu Liu, Tian Liu, Yushou Wu, Yunfei Chi |
| Source: |
European Journal of Medical Research, Vol 30, Iss 1, Pp 1-16 (2025) |
| Publisher Information: |
BMC, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
Burn-hemorrhagic shock combined injury, Organic acids, Amino acids, Matrix and RBC-related proteins, Medicine |
| More Details: |
Abstract Background Burn-hemorrhagic shock combined injury, a severe condition causing complex stress responses and metabolic disturbances that significantly affect clinical outcomes in both military and civilian settings, was modeled in swine to investigate the associated metabolomic and proteomic changes and identify potential biomarkers for disease prognosis. Methods Eight clean-grade adult male Landrace pigs (4–5 months, average weight 60–70 kg) were used to model burn-hemorrhagic shock combined injury. Serum samples collected at 0 h and 2 h post-injury were analyzed using metabolomic and proteomic measurements. The metabolomic and proteomic data were processed through partial least squares–discriminant analysis (PLS–DA) and the KEGG enrichment etc. Furthermore, the integrate analysis of the metabolomic and proteomic data was generalized by canonical correlation discriminant analysis, and the correlation between metabolites and mortality of the swine model was predicted using a multiple linear regression model by Pearson analysis. Results PLS–DA revealed a global shift in each of the metabolomic and proteomic profiles following injury. The levels of 87 signature metabolites including various types of amino acids, fatty acids and acyl-carnitines of different lengths, and many metabolites in the gluconeogenesis, glycolysis, and tricarboxylic acid (TCA) cycle are generally increased (P |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2047-783X |
| Relation: |
https://doaj.org/toc/2047-783X |
| DOI: |
10.1186/s40001-024-02245-0 |
| Access URL: |
https://doaj.org/article/b90b3c71adea4a30bf03ba76e7d7f732 |
| Accession Number: |
edsdoj.b90b3c71adea4a30bf03ba76e7d7f732 |
| Database: |
Directory of Open Access Journals |
