Academic Journal
Systematic transcriptome-wide analysis and validation of tributyltin-induced differential changes in the liver with sex-specific effects
| Title: | Systematic transcriptome-wide analysis and validation of tributyltin-induced differential changes in the liver with sex-specific effects |
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| Authors: | Jinyan Wang, Xin Gu, Pengchen Chen, Sisi Wang, Pan Huang, Yaping Niu, Wenyue Yang, Ziyang Ding, Yanting Liang, Mingjun Shi, Ran Wei, Wei Wang |
| Source: | Ecotoxicology and Environmental Safety, Vol 293, Iss , Pp 117995- (2025) |
| Publisher Information: | Elsevier, 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Environmental pollution LCC:Environmental sciences |
| Subject Terms: | Tributyltin, Liver, Sex-specific, Transcriptome analysis, STAT3, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| More Details: | Background: Tributyltin (TBT), a prevalent environmental antiseptic, contaminates seafood, fish, and drinking water, posing health risks. While TBT’s hepatic toxicity is well-known, its sex-specific effects on liver function remain poorly understood. Methods: To address this gap, a comprehensive analysis was conducted utilizing the Toxicant Exposures and Responses by Genomic and Epigenomic Regulators of Transcription (TaRGET) dataset. Chromatin accessibility changes and transcriptomic alterations were analyzed via ATAC-seq and RNA-seq in liver tissues from TBT-exposed male and female mice. In vitro experiments were performed to validate the key bioinformatic findings. Results: TBT exposure induced significant chromatin accessibility changes and transcriptomic alterations in male liver compared to female counterparts. Notably, Signal transducer and activator of transcription 3 (STAT3) was identified as a central regulator among differentially expressed genes (DEGs) in male liver cells. Functional validation experiments confirmed that TBT-mediated downregulation of STAT3 impaired liver cell function and contributed to increased hepatotoxicity in males. Conclusions: Our study highlights significant sex-dependent differences in TBT-induced hepatotoxicity and identifies STAT3 as a critical mediator in male liver cells, providing a novel perspective on the toxicology of TBT. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0147-6513 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651325003318; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2025.117995 |
| Access URL: | https://doaj.org/article/b8cb508babd9458c8893d301441ebff2 |
| Accession Number: | edsdoj.b8cb508babd9458c8893d301441ebff2 |
| Database: | Directory of Open Access Journals |
| ISSN: | 01476513 |
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| DOI: | 10.1016/j.ecoenv.2025.117995 |
| Published in: | Ecotoxicology and Environmental Safety |
| Language: | English |