| Title: |
Exploring heterogeneous cell population dynamics in different microenvironments by novel analytical strategy based on images |
| Authors: |
Yihong Huang, Zidong Zhou, Tianqi Liu, Shengnan Tang, Xuegang Xin |
| Source: |
npj Systems Biology and Applications, Vol 10, Iss 1, Pp 1-10 (2024) |
| Publisher Information: |
Nature Portfolio, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
Biology (General), QH301-705.5 |
| More Details: |
Abstract Understanding the dynamic states and transitions of heterogeneous cell populations is crucial for addressing fundamental biological questions. High-content imaging provides rich datasets, but it remains increasingly difficult to integrate and annotate high-dimensional and time-resolved datasets to profile heterogeneous cell population dynamics in different microenvironments. Using hepatic stellate cells (HSCs) LX-2 as model, we proposed a novel analytical strategy for image-based integration and annotation to profile dynamics of heterogeneous cell populations in 2D/3D microenvironments. High-dimensional features were extracted from extensive image datasets, and cellular states were identified based on feature profiles. Time-series clustering revealed distinct temporal patterns of cell shape and actin cytoskeleton reorganization. We found LX-2 showed more complex membrane dynamics and contractile systems with an M-shaped actin compactness trend in 3D culture, while they displayed rapid spreading in early 2D culture. This image-based integration and annotation strategy enhances our understanding of HSCs heterogeneity and dynamics in complex extracellular microenvironments. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2056-7189 |
| Relation: |
https://doaj.org/toc/2056-7189 |
| DOI: |
10.1038/s41540-024-00459-w |
| Access URL: |
https://doaj.org/article/b84e3851073e4b5ebd16549e6b9f4f26 |
| Accession Number: |
edsdoj.b84e3851073e4b5ebd16549e6b9f4f26 |
| Database: |
Directory of Open Access Journals |
