Bibliographic Details
| Title: |
4-Thiobenzoic acid-functionalized nanocomposite probes for surface-enhanced raman spectroscopy-based detection of CA19-9 in colorectal adenocarcinoma |
| Authors: |
Yinan Hu, Tao Liu, Bo Guo, Miao Ni, Yanrong Li |
| Source: |
Alexandria Engineering Journal, Vol 119, Iss , Pp 259-266 (2025) |
| Publisher Information: |
Elsevier, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Engineering (General). Civil engineering (General) |
| Subject Terms: |
SERS biosensor, Gold nanoparticles, Tumor marker, Immunoassay, Raman spectroscopy, Engineering (General). Civil engineering (General), TA1-2040 |
| More Details: |
This study presents a novel surface-enhanced Raman spectroscopy (SERS) method for detecting CA19–9 in colorectal adenocarcinoma using 4-thiobenzoic acid (4-TBA) functionalized gold nanoparticles (nanospheres). The optimized SERS probes exhibited a maximum enhancement at pH 7 and 50 μM 4-TBA concentration, with stable signal achieved after 2 hours of incubation. The assay demonstrated a wide dynamic range of 1–100 U/mL, with a lower limit of quantification of 1.0 U/mL. Excellent selectivity was observed against common serum interferents, with normalized SERS intensities within 10 % of CA19–9 alone. Recovery studies in spiked serum samples yielded results ranging from 94.5 % to 105.2 %, indicating minimal matrix effects. Clinical validation using 70 serum samples (50 colorectal adenocarcinoma patients, 20 healthy volunteers) showed strong agreement with a commercial ELISA kit (Pearson's r = 0.987, p |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1110-0168 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1110016825001498; https://doaj.org/toc/1110-0168 |
| DOI: |
10.1016/j.aej.2025.01.122 |
| Access URL: |
https://doaj.org/article/ab7f1e554890480e8ba70d16641892fe |
| Accession Number: |
edsdoj.b7f1e554890480e8ba70d16641892fe |
| Database: |
Directory of Open Access Journals |
