Bibliographic Details
| Title: |
Fluidic Automation of Nitrate and Nitrite Bioassays in Whole Blood by Dissolvable-Film Based Centrifugo-Pneumatic Actuation |
| Authors: |
Jens Ducrée, Robert Gorkin, Robert Burger, Di-Sien S. Chan, Jennifer Gaughran, Charles E. Nwankire |
| Source: |
Sensors, Vol 13, Iss 9, Pp 11336-11349 (2013) |
| Publisher Information: |
MDPI AG, 2013. |
| Publication Year: |
2013 |
| Collection: |
LCC:Chemical technology |
| Subject Terms: |
dissolvable film, sacrificial valve, reagent storage, centrifugal, microfluidics, automation, Chemical technology, TP1-1185 |
| More Details: |
This paper demonstrates the full centrifugal microfluidic integration and automation of all liquid handling steps of a 7-step fluorescence-linked immunosorbent assay (FLISA) for quantifying nitrate and nitrite levels in whole blood within about 15 min. The assay protocol encompasses the extraction of metered plasma, the controlled release of sample and reagents (enzymes, co-factors and fluorescent labels), and incubation and detection steps. Flow control is implemented by a rotationally actuated dissolvable film (DF) valving scheme. In the valves, the burst pressure is primarily determined by the radial position, geometry and volume of the valve chamber and its inlet channel and can thus be individually tuned over an extraordinarily wide range of equivalent spin rates between 1,000 RPM and 5,500 RPM. Furthermore, the vapour barrier properties of the DF valves are investigated in this paper in order to further show the potential for commercially relevant on-board storage of liquid reagents during shelf-life of bioanalytical, ready-to-use discs. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1424-8220 |
| Relation: |
http://www.mdpi.com/1424-8220/13/9/11336; https://doaj.org/toc/1424-8220 |
| DOI: |
10.3390/s130911336 |
| Access URL: |
https://doaj.org/article/ab7b1ccd15384223b458585df438545b |
| Accession Number: |
edsdoj.b7b1ccd15384223b458585df438545b |
| Database: |
Directory of Open Access Journals |
