| Title: |
Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City |
| Authors: |
Zahraa Mohammed Wahib, Sahira Nsayef Muslim, Farha A. Ali Shafi |
| Source: |
Iraqi Journal of Industrial Research, Vol 11, Iss 3 (2024) |
| Publisher Information: |
Corporation of Research and Industrial Development, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Special industries and trades
LCC:Industrial engineering. Management engineering |
| Subject Terms: |
Gram-negative bacteria, L-methioninase, Optimization conditions, Special industries and trades, HD9000-9999, Industrial engineering. Management engineering, T55.4-60.8 |
| More Details: |
L-methioninase, a pyridoxal 5′-phosphate dependent enzyme that catalyze degradation of L-methionine to ammonia, methanethiol and α -ketobutyrate. L-methioninase had received a lot of interest for its importance, so to improve the production of L-methioninase, media optimization was done. In this study, clinical isolates of gram-negative bacteria have been collected from several hospitals located in Baghdad city, these isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii and Serratia marcescens. The screening for detecting L-methioninase production was performed by semi-quantitative and quantitative analysis, the results revealed that out of all obtained isolates, 31(41.33%) were L-methioninase producers from semi-quantitative screening while by quantitative method only 16 isolates out of these 31 isolates revealed specific activity ranged from 0.19 to 1.15 U/mg and the maximum specific activity was for E. coli U8, which was chosen as best producer isolates. The L-methioninase activity reached its maximum level when E. coli U8 was cultivated with the best conditions, which is consisted of using modified mineral salt M9 broth medium supplemented with L-methionine mixed with galactose (2 g/L) as carbon source and L-glutamine (1.5 g/L) and incubated at 37°C for 48 hours at pH 7. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2788-712X |
| Relation: |
http://ijoir.gov.iq/ijoir/index.php/jou/article/view/494; https://doaj.org/toc/2788-712X |
| DOI: |
10.53523/ijoirVol11I3ID494 |
| Access URL: |
https://doaj.org/article/ab782d8e711f482b81eb270ca70d76c6 |
| Accession Number: |
edsdoj.b782d8e711f482b81eb270ca70d76c6 |
| Database: |
Directory of Open Access Journals |
