| Title: |
Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection |
| Authors: |
Paula Piñero, Francisco M Marco De La Calle, Lydia Horndler, Balbino Alarcón, Marisol Uribe Barrientos, Héctor Sarmiento, Fabián Tarín |
| Source: |
Scientific Reports, Vol 11, Iss 1, Pp 1-5 (2021) |
| Publisher Information: |
Nature Portfolio, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
Abstract SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2045-2322 |
| Relation: |
https://doaj.org/toc/2045-2322 |
| DOI: |
10.1038/s41598-021-90054-4 |
| Access URL: |
https://doaj.org/article/b6c6d37fd4a044d496e9fc4b7cf56f4f |
| Accession Number: |
edsdoj.b6c6d37fd4a044d496e9fc4b7cf56f4f |
| Database: |
Directory of Open Access Journals |
