Characterization of SNEDDS Formulated with Single Bulb Garlic and its Anti-Inflammatory Effect on 3T3-L1
| Title: | Characterization of SNEDDS Formulated with Single Bulb Garlic and its Anti-Inflammatory Effect on 3T3-L1 |
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| Authors: | Sri Rahayu Lestari, Sunaryono Sunaryono, Hendra Susanto, Hidajah Rachmawati, Rony Irawanto, Dewi Sekar Miasih, Alif Rosyidah El Baroroh, Yuslinda Annisa, Muhammad Sasmito Djati |
| Source: | Hayati Journal of Biosciences, Vol 32, Iss 3 (2025) |
| Publisher Information: | Bogor Agricultural University, 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Biology (General) |
| Subject Terms: | Biology (General), QH301-705.5 |
| More Details: | Inflammation is an early symptom of a disease that reduces the level of health. Single-bulb garlic (Allium sativum L.) is used medicinally as a plant with a broad pharmacological effect, especially anti-inflammatory activity. Self-nanoemulsifying drug delivery systems (SNEDDS) have offered opportunities to improve drug delivery. The current study aimed to characterize SNEDDS-single bulb garlic extract (SBGE) and determine its potential as an anti-inflammatory agent in 3T3-L1 cells. SNEDDS was formulated from tween-80, glycerol, canola oil, and SBGE. The formula characterization is done using droplet size, polydispersity index, zeta potential, physical stability test, and antioxidant assay. The cytotoxicity test of SNEDDS-SBGE was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The anti-inflammatory activity was examined using 3T3-L1 cell methylglyoxal (MG) induction, and the expression of cytokines was measured using immunocytochemistry (ICC). The SNEDDS-SBGE had a nanoemulsion size of 42.30±1.39 nm, 0.6±0.03 for the polydispersion index, and -22.63±0.75 mV for the zeta potential. SNEDDS-SBGE was physically stable and had a high antioxidant level (47.579±8.017 %). SNEDDS-SBGE exhibited no toxic effect on 3T3-L1 cells. The administration of 62.5 µg/ml and 125 µg/ml SNEDDS-SBGE could inhibit (p |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Indonesian |
| ISSN: | 1978-3019 2086-4094 |
| Relation: | https://journal.ipb.ac.id/index.php/hayati/article/view/59039; https://doaj.org/toc/1978-3019; https://doaj.org/toc/2086-4094 |
| DOI: | 10.4308/hjb.32.3.793-804 |
| Access URL: | https://doaj.org/article/b5d224344062433aadb4f7069b49ab5d |
| Accession Number: | edsdoj.b5d224344062433aadb4f7069b49ab5d |
| Database: | Directory of Open Access Journals |
| ISSN: | 19783019 20864094 |
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| DOI: | 10.4308/hjb.32.3.793-804 |
| Published in: | Hayati Journal of Biosciences |
| Language: | English Indonesian |