Comparing the clinical significance and antigen specificity of insulinoma‐associated antigen‐2 autoantibodies between radioimmunoassay and enzyme‐linked immunosorbent assay in Japanese patients with type 1 diabetes
| Title: | Comparing the clinical significance and antigen specificity of insulinoma‐associated antigen‐2 autoantibodies between radioimmunoassay and enzyme‐linked immunosorbent assay in Japanese patients with type 1 diabetes |
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| Authors: | Eiji Kawasaki, Akira Shimada, Akihisa Imagawa, Norio Abiru, Takuya Awata, Yoichi Oikawa, Haruhiko Osawa, Yumiko Kawabata, Junji Kozawa, Tetsuro Kobayashi, Kazuma Takahashi, Daisuke Chujo, Tomoyasu Fukui, Junnosuke Miura, Kazuki Yasuda, Hisafumi Yasuda, Hiroshi Kajio, Toshiaki Hanafusa, Hiroshi Ikegami, the Committee of type 1 diabetes, Japan Diabetes Society |
| Source: | Journal of Diabetes Investigation, Vol 14, Iss 1, Pp 58-66 (2023) |
| Publisher Information: | Wiley, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Diseases of the endocrine glands. Clinical endocrinology |
| Subject Terms: | Autoantibodies, Autoantigen, Insulinoma‐associated antigen‐2, Type 1 diabetes, Diseases of the endocrine glands. Clinical endocrinology, RC648-665 |
| More Details: | Abstract Aims/Introduction This study aimed to investigate the clinical significance and antigen specificity of autoantibodies to insulinoma‐associated antigen‐2 (IA‐2A) by radioimmunoassay (RIA; IA‐2A‐RIA) and enzyme‐linked immunosorbent assay (ELISA; IA‐2A‐ELISA) in Japanese patients with type 1 diabetes. Materials and Methods A total of 338 type 1 diabetic patients were enrolled, including 38 fulminant type 1 diabetes, 168 acute‐onset type 1 diabetes and 137 slowly‐progressive type 1 diabetes (SPIDDM). The concordance, correlation of autoantibody titer, and the relationship between IA‐2A and progression to the insulin‐deficient state were examined. Also, competitive assay was used to examine the antigen specificity. Results The prevalence of IA‐2A‐ELISA was 4–5% lower than that of IA‐2A‐RIA in both the acute‐onset type 1 diabetes and SPIDDM, but the diagnostic sensitivities of both subtypes, when measured in combination with glutamic acid decarboxylase autoantibody, were comparable. The diagnosis of type 1 diabetes using either the RIA or ELISA methods showed substantial agreement with the exponential correlation of autoantibody titers detected by RIA and ELISA. Among the SPIDDM patients, the fasting C‐peptide for IA‐2A‐positive cases by ELISA, but not the RIA method, was significantly lower than in the negative cases (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2040-1124 2040-1116 |
| Relation: | https://doaj.org/toc/2040-1116; https://doaj.org/toc/2040-1124 |
| DOI: | 10.1111/jdi.13910 |
| Access URL: | https://doaj.org/article/b5d09b3b2ef946e3ad57d361fe587d35 |
| Accession Number: | edsdoj.b5d09b3b2ef946e3ad57d361fe587d35 |
| Database: | Directory of Open Access Journals |
| ISSN: | 20401124 20401116 |
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| DOI: | 10.1111/jdi.13910 |
| Published in: | Journal of Diabetes Investigation |
| Language: | English |