Academic Journal
Overexpression of Vitis GRF4-GIF1 improves regeneration efficiency in diploid Fragaria vesca Hawaii 4
| Title: | Overexpression of Vitis GRF4-GIF1 improves regeneration efficiency in diploid Fragaria vesca Hawaii 4 |
|---|---|
| Authors: | Esther Rosales Sanchez, R. Jordan Price, Federico Marangelli, Kirsty McLeary, Richard J. Harrison, Anindya Kundu |
| Source: | Plant Methods, Vol 20, Iss 1, Pp 1-15 (2024) |
| Publisher Information: | BMC, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Plant culture LCC:Biology (General) |
| Subject Terms: | Strawberry, Regeneration, Transformation, GRF4-GIF1 chimera, Leaf development, Cytokinin, Plant culture, SB1-1110, Biology (General), QH301-705.5 |
| More Details: | Abstract Background Plant breeding played a very important role in transforming strawberries from being a niche crop with a small geographical footprint into an economically important crop grown across the planet. But even modern marker assisted breeding takes a considerable amount of time, over multiple plant generations, to produce a plant with desirable traits. As a quicker alternative, plants with desirable traits can be raised through tissue culture by doing precise genetic manipulations. Overexpression of morphogenic regulators previously known for meristem development, the transcription factors Growth-Regulating Factors (GRFs) and the GRF-Interacting Factors (GIFs), provided an efficient strategy for easier regeneration and transformation in multiple crops. Results We present here a comprehensive protocol for the diploid strawberry Fragaria vesca Hawaii 4 (strawberry) regeneration and transformation under control condition as compared to ectopic expression of different GRF4-GIF1 chimeras from different plant species. We report that ectopic expression of Vitis vinifera VvGRF4-GIF1 provides significantly higher regeneration efficiency during re-transformation over wild-type plants. On the other hand, deregulated expression of miRNA resistant version of VvGRF4-GIF1 or Triticum aestivum (wheat) TaGRF4-GIF1 resulted in abnormalities. Transcriptomic analysis between the different chimeric GRF4-GIF1 lines indicate that differential expression of FvExpansin might be responsible for the observed pleiotropic effects. Similarly, cytokinin dehydrogenase/oxygenase and cytokinin responsive response regulators also showed differential expression indicating GRF4-GIF1 pathway playing important role in controlling cytokinin homeostasis. Conclusion Our data indicate that ectopic expression of Vitis vinifera VvGRF4-GIF1 chimera can provide significant advantage over wild-type plants during strawberry regeneration without producing any pleiotropic effects seen for the miRNA resistant VvGRF4-GIF1 or TaGRF4-GIF1. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1746-4811 |
| Relation: | https://doaj.org/toc/1746-4811 |
| DOI: | 10.1186/s13007-024-01270-8 |
| Access URL: | https://doaj.org/article/b5b0f93d020743f0a5418d79f4f72f05 |
| Accession Number: | edsdoj.b5b0f93d020743f0a5418d79f4f72f05 |
| Database: | Directory of Open Access Journals |
| Full text is not displayed to guests. | Login for full access. |
| ISSN: | 17464811 |
|---|---|
| DOI: | 10.1186/s13007-024-01270-8 |
| Published in: | Plant Methods |
| Language: | English |