Minimally Invasive Transthoracic Intramyocardial Cellular Transplantation Under Echocardiographic Guidance for Myocardial Impairment
| Title: | Minimally Invasive Transthoracic Intramyocardial Cellular Transplantation Under Echocardiographic Guidance for Myocardial Impairment |
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| Authors: | Sulei Li, Bo Tao, Junsong Liu, Dongyue Liu, Yujia Wang, Jibin Zhang, Lei Tian, Baixuan Xu, Yan Fang, Yabin Wang, Xin Lou, Jinming Zhang, Feng Lan, Feng Cao, Hanjia Gao, Xiaoxia Fu |
| Source: | Cardiology Discovery, Vol 4, Iss 4, Pp 260-273 (2024) |
| Publisher Information: | Wolters Kluwer Health/LWW, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Diseases of the circulatory (Cardiovascular) system |
| Subject Terms: | Diseases of the circulatory (Cardiovascular) system, RC666-701 |
| More Details: | Abstract. Objective:. To explore the approach of minimally invasive transthoracic intramyocardial cellular transplantation under echocardiographic guidance to promote ischemic myocardial repair in a preclinical big-animal study. Methods:. Female Guangxi Bama miniature pigs (weight: 25–30 kg) were randomly allocated into the sham group, untreated myocardial infarction (MI) group (MI group), the MI and surgical intramyocardial injection (SIM) group (MI-SIM group), and the MI and transthoracic echocardiography-guided percutaneous intramyocardial injection (TTEPIM) group (MI-TTEPIM group) (n = 4 each) using a lottery method. A swine MI model was established in the 3 groups excluding the sham group, and human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CM) labeled with the herpes simplex virus type-1 thymidine kinase reporter gene (hiPS-CMTK+) were transplanted by SIM in MI-SIM group and TTEPIM in MI-TTEPIM group. The operation time, postoperative recovery time of animals and volume of blood loss were collected for comparison between MI-SIM group and MI-TTEPIM group. 9-(4-[18F] fluoro-3-(hydroxymethyl) butyl) guanine positron emission tomography/computed tomography imaging was performed to track the hiPS-CMTK+in vivo. Cardiac function and morphology were evaluated by echocardiography. Results:. The operation time and postoperative recovery time of MI-TTEPIM group were significantly shorter than those of MI-SIM group ((28.3 ± 3.6) min vs. (97.0 ± 6.7) min, P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2096-952X 00000000 |
| Relation: | http://journals.lww.com/10.1097/CD9.0000000000000142; https://doaj.org/toc/2096-952X |
| DOI: | 10.1097/CD9.0000000000000142 |
| Access URL: | https://doaj.org/article/b509d8f1c67841a085a3d3e6c4d17516 |
| Accession Number: | edsdoj.b509d8f1c67841a085a3d3e6c4d17516 |
| Database: | Directory of Open Access Journals |
| ISSN: | 2096952X 00000000 |
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| DOI: | 10.1097/CD9.0000000000000142 |
| Published in: | Cardiology Discovery |
| Language: | English |