| Title: |
Characterization of immune populations in the tumor microenvironment of diffuse midline glioma orthotopic mouse models by flow cytometry |
| Authors: |
Iker Ausejo-Mauleon, Sara Labiano, Marta M. Alonso |
| Source: |
STAR Protocols, Vol 5, Iss 1, Pp 102803- (2024) |
| Publisher Information: |
Elsevier, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Science (General) |
| Subject Terms: |
Flow Cytometry, Cancer, Immunology, Science (General), Q1-390 |
| More Details: |
Summary: The immune response is a fundamental process in the treatment of solid tumors. Here, we present a protocol for implanting diffuse midline glioma cells in the brain of immune-competent mice and characterizing the different immune populations in the tumor microenvironment in a flow cytometry panel. We describe steps for processing of brain tumors, isolating the immune cells, and subsequent staining with antibodies for flow cytometry. We then detail procedures for implementing the gating strategy.For complete details on the use and execution of this protocol, please refer to Ausejo-Mauleon et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166723007700; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2023.102803 |
| Access URL: |
https://doaj.org/article/cb504450ac8f410193a03eda74f9847a |
| Accession Number: |
edsdoj.b504450ac8f410193a03eda74f9847a |
| Database: |
Directory of Open Access Journals |
