Bibliographic Details
| Title: |
Triage of HR-HPV positive women with minor cytological abnormalities: a comparison of mRNA testing, HPV DNA testing, and repeat cytology using a 4-year follow-up of a population-based study. |
| Authors: |
Maria Persson, K Miriam Elfström, Sophia Brismar Wendel, Elisabete Weiderpass, Sonia Andersson |
| Source: |
PLoS ONE, Vol 9, Iss 2, p e90023 (2014) |
| Publisher Information: |
Public Library of Science (PLoS), 2014. |
| Publication Year: |
2014 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
ObjectiveExpression of the viral E6/E7 oncogenes of high-risk human papillomaviruses (HR-HPV) is necessary for malignant conversion and maintenance in cervical tissue. In order to determine whether HR-HPV E6/E7 mRNA testing more effectively predicts precancerous lesions and invasive cervical cancer than HR-HPV DNA testing, we aimed to compare triage using HR-HPV E6/E7 mRNA testing by APTIMA HPV Assay (APTIMA) to HPV16 DNA testing, HPV16/18 DNA testing, and repeat cytology.MethodsLiquid-based (PreservCyt) cell samples were obtained from HR-HPV-positive women diagnosed with atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesions (LSIL) within the framework of the population-based cervical cancer screening program in Stockholm, Sweden. Samples were tested for HR-HPV E6/E7 mRNA by APTIMA (Gene-Probe Inc., San Diego, CA, USA). Women were followed up for 4 years after the index cytology via medical and laboratory records, and the Stockholm Oncology Center.ResultsNine of 25 (36%) women in the ASCUS group, and 64 of 180 (36%) women in the LSIL group developed cervical intraepithelial neoplasia (CIN) grade 2 or worse during 4 years of follow-up. 162 (74%) women were APTIMA-positive, and APTIMA had the highest sensitivity to predict CIN2 or worse and CIN3 or worse in the ASCUS (77.8% and 100%) and LSIL (78.1 and 75.8%) groups, although specificity was insufficient (ConclusionThe results of this population-based study with comprehensive follow-up support the use of APTIMA as a triage test for women with ASCUS. More focused investigation is required for women with LSIL. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090023&type=printable; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0090023&type=printable |
| DOI: |
10.1371/journal.pone.0090023 |
| Access URL: |
https://doaj.org/article/b4d5ae0d6d9740deb0197407faa92640 |
| Accession Number: |
edsdoj.b4d5ae0d6d9740deb0197407faa92640 |
| Database: |
Directory of Open Access Journals |
