Comparative Study of Expansion and Proliferation of Adult Mice Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue
| Title: | Comparative Study of Expansion and Proliferation of Adult Mice Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue |
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| Authors: | Mahfoodha Abbas Umran, Marwa Ibrahim Salman |
| Source: | مجلة مركز بحوث التقنيات الاحيائية, Vol 10, Iss 2 (2016) |
| Publisher Information: | Al-Nahrain University/ Biotechnology Research Center, 2016. |
| Publication Year: | 2016 |
| Collection: | LCC:Biotechnology LCC:Biology (General) |
| Subject Terms: | MSCs, cell therapy, adult mesenchymal stem cells, bone marrow mesenchymal, stemcells, adipose-derived stem cells., Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5 |
| More Details: | The importance of Mesenchymal stem cells (MSCs) represents a favorable tool for new clinical concepts in supporting tissue engineering and cellular therapy. Bone marrow (BM) was considered important source contain mesenchymal stem cells .Another promising source of MSCs is adipose tissue (AT). MSCs derived from these sources compared regarding morphology, the success rate of isolating MSCs, expansion potential by rate of colony forming and immune phenotype. The obtained results from this study showed no obvious considerable differences concerning the morphology and immune phenotype of the MSCs derived from these sources were obvious. Differences observed concerning to the success rate of isolating MSCs, which was approximately more than 90% for BM, while it reached about 70% for AT after seven days of culturing, as well as the rate of colony forming was lower in AT cells in comparison to that obtained in BM at the same period. However, AT-MSCs could be required longest time to complete monolayer confluence, whereas BM-MSCs had the shortest proliferation period. Cells from both sources determined according to immunohistochemistry by CD105+ and CD34.¯ Conclusions revealed that MSCs can easily and successfully obtained from bone marrow and adipose tissues, and both tissues appears suitable sources of stem cells for potential use in regenerative medicine, repairing damaged tissue nevertheless the BM-MSCs more effectual in expansion and proliferation. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1815-1140 2708-1370 44923368 |
| Relation: | https://www.jobrc.org/index.php/jobrc/article/view/481; https://doaj.org/toc/1815-1140; https://doaj.org/toc/2708-1370 |
| DOI: | 10.24126/jobrc.2016.10.2.481 |
| Access URL: | https://doaj.org/article/b449233681be4ffbb3264183de06ba3e |
| Accession Number: | edsdoj.b449233681be4ffbb3264183de06ba3e |
| Database: | Directory of Open Access Journals |
| ISSN: | 18151140 27081370 44923368 |
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| DOI: | 10.24126/jobrc.2016.10.2.481 |
| Published in: | مجلة مركز بحوث التقنيات الاحيائية |
| Language: | English |