Academic Journal
Noninvasive optical and nuclear imaging of Staphylococcus-specific infection with a human monoclonal antibody-based probe
| Title: | Noninvasive optical and nuclear imaging of Staphylococcus-specific infection with a human monoclonal antibody-based probe |
|---|---|
| Authors: | Francisco Romero Pastrana, John M. Thompson, Marjolein Heuker, Hedzer Hoekstra, Carly A. Dillen, Roger V. Ortines, Alyssa G. Ashbaugh, Julie E. Pickett, Matthijs D. Linssen, Nicholas M. Bernthal, Kevin P. Francis, Girbe Buist, Marleen van Oosten, Gooitzen M. van Dam, Daniel L. J. Thorek, Lloyd S. Miller, Jan Maarten van Dijl |
| Source: | Virulence, Vol 9, Iss 1, Pp 262-272 (2018) |
| Publisher Information: | Taylor & Francis Group, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Infectious and parasitic diseases |
| Subject Terms: | human monoclonal antibody, immunodominant staphylococcal antigen A, IsaA, PET, 89Zr, Staphylococcus aureus, Infectious and parasitic diseases, RC109-216 |
| More Details: | Staphylococcus aureus infections are a major threat in healthcare, requiring adequate early-stage diagnosis and treatment. This calls for novel diagnostic tools that allow noninvasive in vivo detection of staphylococci. Here we performed a preclinical study to investigate a novel fully-human monoclonal antibody 1D9 that specifically targets the immunodominant staphylococcal antigen A (IsaA). We show that 1D9 binds invariantly to S. aureus cells and may further target other staphylococcal species. Importantly, using a human post-mortem implant model and an in vivo murine skin infection model, preclinical feasibility was demonstrated for 1D9 labeled with the near-infrared fluorophore IRDye800CW to be applied for direct optical imaging of in vivo S. aureus infections. Additionally, 89Zirconium-labeled 1D9 could be used for positron emission tomography imaging of an in vivo S. aureus thigh infection model. Our findings pave the way towards clinical implementation of targeted imaging of staphylococcal infections using the human monoclonal antibody 1D9. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2150-5594 2150-5608 21505594 |
| Relation: | https://doaj.org/toc/2150-5594; https://doaj.org/toc/2150-5608 |
| DOI: | 10.1080/21505594.2017.1403004 |
| Access URL: | https://doaj.org/article/b3948013660441b29da4311a15413d71 |
| Accession Number: | edsdoj.b3948013660441b29da4311a15413d71 |
| Database: | Directory of Open Access Journals |
| Full text is not displayed to guests. | Login for full access. |
| ISSN: | 21505594 21505608 |
|---|---|
| DOI: | 10.1080/21505594.2017.1403004 |
| Published in: | Virulence |
| Language: | English |