Bibliographic Details
| Title: |
Dietary Compound Isoliquiritigenin, an Antioxidant from Licorice, Suppresses Triple-Negative Breast Tumor Growth via Apoptotic Death Program Activation in Cell and Xenograft Animal Models |
| Authors: |
Po-Han Lin, Yi-Fen Chiang, Tzong-Ming Shieh, Hsin-Yuan Chen, Chun-Kuang Shih, Tong-Hong Wang, Kai-Lee Wang, Tsui-Chin Huang, Yong-Han Hong, Sing-Chung Li, Shih-Min Hsia |
| Source: |
Antioxidants, Vol 9, Iss 3, p 228 (2020) |
| Publisher Information: |
MDPI AG, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Therapeutics. Pharmacology |
| Subject Terms: |
isoliquiritigenin (isl), triple-negative breast cancer, apoptosis, autophagy, Therapeutics. Pharmacology, RM1-950 |
| More Details: |
Patients with triple-negative breast cancer have few therapeutic strategy options. In this study, we investigated the effect of isoliquiritigenin (ISL) on the proliferation of triple-negative breast cancer cells. We found that treatment with ISL inhibited triple-negative breast cancer cell line (MDA-MB-231) cell growth and increased cytotoxicity. ISL reduced cell cycle progression through the reduction of cyclin D1 protein expression and increased the sub-G1 phase population. The ISL-induced apoptotic cell population was observed by flow cytometry analysis. The expression of Bcl-2 protein was reduced by ISL treatment, whereas the Bax protein level increased; subsequently, the downstream signaling molecules caspase-3 and poly ADP-ribose polymerase (PARP) were activated. Moreover, ISL reduced the expression of total and phosphorylated mammalian target of rapamycin (mTOR), ULK1, and cathepsin B, whereas the expression of autophagic-associated proteins p62, Beclin1, and LC3 was increased. The decreased cathepsin B cause the p62 accumulation to induce caspase-8 mediated apoptosis. In vivo studies further showed that preventive treatment with ISL could inhibit breast cancer growth and induce apoptotic and autophagic-mediated apoptosis cell death. Taken together, ISL exerts an effect on the inhibition of triple-negative MDA-MB-231 breast cancer cell growth through autophagy-mediated apoptosis. Therefore, future studies of ISL as a supplement or alternative therapeutic agent for clinical trials against breast cancer are warranted. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2076-3921 |
| Relation: |
https://www.mdpi.com/2076-3921/9/3/228; https://doaj.org/toc/2076-3921 |
| DOI: |
10.3390/antiox9030228 |
| Access URL: |
https://doaj.org/article/b34123629d694164a3533eafed8c0500 |
| Accession Number: |
edsdoj.b34123629d694164a3533eafed8c0500 |
| Database: |
Directory of Open Access Journals |
