Bibliographic Details
| Title: |
Molecular recognition of Escherichia coli R1-type core lipooligosaccharide by DC-SIGN |
| Authors: |
Ferran Nieto-Fabregat, Angela Marseglia, Michel Thépaut, Jean-Philippe Kleman, Massilia Abbas, Aline Le Roy, Christine Ebel, Meriem Maalej, Jean-Pierre Simorre, Cedric Laguri, Antonio Molinaro, Alba Silipo, Franck Fieschi, Roberta Marchetti |
| Source: |
iScience, Vol 27, Iss 2, Pp 108792- (2024) |
| Publisher Information: |
Elsevier, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Science |
| Subject Terms: |
Microbiology, Structural biology, Science |
| More Details: |
Summary: Due to their ability to recognize carbohydrate structures, lectins emerged as potential receptors for bacterial lipopolysaccharides (LPS). Despite growing interest in investigating the association between host receptor lectins and exogenous glycan ligands, the molecular mechanisms underlying bacterial recognition by human lectins are still not fully understood. We contributed to fill this gap by unveiling the molecular basis of the interaction between the lipooligosaccharide of Escherichia coli and the dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin (DC-SIGN). Specifically, a combination of different techniques, including fluorescence microscopy, surface plasmon resonance, NMR spectroscopy, and computational studies, demonstrated that DC-SIGN binds to the purified deacylated R1 lipooligosaccharide mainly through the recognition of its outer core pentasaccharide, which acts as a crosslinker between two different tetrameric units of DC-SIGN. Our results contribute to a better understanding of DC-SIGN-LPS interaction and may support the development of pharmacological and immunostimulatory strategies for bacterial infections, prevention, and therapy. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004224000130; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2024.108792 |
| Access URL: |
https://doaj.org/article/b0db4208477b44db88da22e26245f019 |
| Accession Number: |
edsdoj.b0db4208477b44db88da22e26245f019 |
| Database: |
Directory of Open Access Journals |
