Identification of potential drugs for treatment of hepatic lipidosis in cats using an in vitro feline liver organoid system
| Title: | Identification of potential drugs for treatment of hepatic lipidosis in cats using an in vitro feline liver organoid system |
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| Authors: | Maya W. Haaker, Hedwig S. Kruitwagen, Arie B. Vaandrager, Martin Houweling, Louis C. Penning, Martijn R. Molenaar, Monique E. vanWolferen, Loes A. Oosterhoff, Bart Spee, J. Bernd Helms |
| Source: | Journal of Veterinary Internal Medicine, Vol 34, Iss 1, Pp 132-138 (2020) |
| Publisher Information: | Wiley, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Veterinary medicine |
| Subject Terms: | AICAR, diacylglycerol O‐acyltransferase 1 inhibitor, hepatic organoids, perilipin 2, steatosis, Veterinary medicine, SF600-1100 |
| More Details: | Abstract Background Hepatic lipidosis is increasing in incidence in the Western world, with cats being particularly sensitive. When cats stop eating and start utilizing their fat reserves, free fatty acids (FFAs) increase in blood, causing an accumulation of triacylglycerol (TAG) in the liver. Objective Identifying potential new drugs that can be used to treat hepatic lipidosis in cats using a feline hepatic organoid system. Animals Liver organoids obtained from 6 cats. Methods Eight different drugs were tested, and the 2 most promising were further studied using a quantitative TAG assay, lipid droplet staining, and qPCR. Results Both T863 (a diacylglycerol O‐acyltransferase 1 [DGAT1] inhibitor) and 5‐aminoimidazole‐4‐carboxamide 1‐β‐D‐ribofuranoside (AICAR; an adenosine monophosphate kinase activator) decreased TAG accumulation by 55% (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1939-1676 0891-6640 |
| Relation: | https://doaj.org/toc/0891-6640; https://doaj.org/toc/1939-1676 |
| DOI: | 10.1111/jvim.15670 |
| Access URL: | https://doaj.org/article/9e1ab320d6b74ca9bb639a32d61656eb |
| Accession Number: | edsdoj.9e1ab320d6b74ca9bb639a32d61656eb |
| Database: | Directory of Open Access Journals |
| ISSN: | 19391676 08916640 |
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| DOI: | 10.1111/jvim.15670 |
| Published in: | Journal of Veterinary Internal Medicine |
| Language: | English |