Bibliographic Details
| Title: |
Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein |
| Authors: |
Bahareh Kordi, Amir Fotovati, Hamidreza Majidiani, Hadi Fazel, Esfandiar Azizi, Morteza Shams, Amir Abaszadeh |
| Source: |
Informatics in Medicine Unlocked, Vol 42, Iss , Pp 101333- (2023) |
| Publisher Information: |
Elsevier, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Computer applications to medicine. Medical informatics |
| Subject Terms: |
Cryptosporidium parvum, SGA, Multi-epitope vaccine, Computer applications to medicine. Medical informatics, R858-859.7 |
| More Details: |
Cryptosporidiosis is a zoonotic parasitic infection causing diarrhea in humans and farm animals. The present study was done to computationally analyze the Cryptosporidium parvum sporozoite glycoprotein antigen (SGA) regarding immunogenic epitopes, as a potential vaccine candidate. Physico-chemical properties, solubility, antigenicity, allergenicity, signal peptide, transmembrane domain and post-translational modifications (PTMs) along with secondary and tertiary structure analyses were predicted. Also, linear B-cell epitopes, cytotoxic T-lymphocyte (CTL; human, cattle) and helper T-lymphocyte (HTL; human) epitopes were forecasted. The molecular weight of this 265-residue protein was 27.14 kDa, with hydrophilicity (GRAVY: 0.700), high solubility (0.892), and aliphatic index (46.83). No signal peptide and transmembrane domain was predicted, and the most abundant PTMs were phosphorylation (n = 68) and O-glycosylation (n = 53). Coils were dominant in secondary structure analysis, and the tertiary model refinement showed enhancement in the refined than crude model. Only 4 shared B-cell epitopes among three web servers (ABCpred, BepiPred 2.0, and SVMTriP) were shown to be antigenic, non-allergenic, with good water solubility. Also, five potent CTL epitopes in humans and two in cattle were predicted. Notably, four HTL epitopes were found as inducers of IFN-γ, IL-4 or both. In conclusion, our results demonstrated several immunogenic epitopes in this protein, which could be directed towards multi-epitope vaccine design. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2352-9148 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S235291482300179X; https://doaj.org/toc/2352-9148 |
| DOI: |
10.1016/j.imu.2023.101333 |
| Access URL: |
https://doaj.org/article/9d631861a5404693b3bed2df4728cca7 |
| Accession Number: |
edsdoj.9d631861a5404693b3bed2df4728cca7 |
| Database: |
Directory of Open Access Journals |
