Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction
| Title: | Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction |
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| Authors: | Ekta Kamra, Netrapal Singh, Anish Khan, Jaideep Singh, Meenakshi Chauhan, Hemant Kamal, Promod K Mehta |
| Source: | BioTechniques, Vol 73, Iss 1, Pp 47-57 (2022) |
| Publisher Information: | Taylor & Francis Group, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Biology (General) |
| Subject Terms: | diagnosis, gel-based LAMP, GUTB, HNB dye, IS6110, M-PCR, Biology (General), QH301-705.5 |
| More Details: | A multitargeted loop-mediated isothermal amplification (MT-LAMP) assay targeting mpt64 (Rv1980c) and IS6110 was designed to diagnose genitourinary tuberculosis (GUTB) cases. While assessing gel-based, hydroxynaphthol blue (HNB) and SYBR Green I MT-LAMP assays on GUTB specimens (n = 28) in a pilot study, both gel-based/SYBR Green I assays exhibited better sensitivity than HNB LAMP. Since SYBR Green MT-LAMP is easier to perform compared with a gel-based assay, a higher number of GUTB specimens (n = 55) were evaluated by SYBR Green MT-LAMP, wherein 85.5% sensitivity and 94.4% specificity (n = 36) were obtained. Moreover, the sensitivity attained by MT-LAMP was significantly higher (p |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1940-9818 0736-6205 |
| Relation: | https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818 |
| DOI: | 10.2144/btn-2022-0027 |
| Access URL: | https://doaj.org/article/d9ae13f0382e465a90e42adab3fd2061 |
| Accession Number: | edsdoj.9ae13f0382e465a90e42adab3fd2061 |
| Database: | Directory of Open Access Journals |
| ISSN: | 19409818 07366205 |
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| DOI: | 10.2144/btn-2022-0027 |
| Published in: | BioTechniques |
| Language: | English |