Academic Journal
View in EDS

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Bibliographic Details
Title: Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters
Authors: Juliana Schons Gularte, Roana de Oliveira Hansen, Meriane Demoliner, Jacek Fiutowski, Ana Karolina Antunes Eisen, Fagner Henrique Heldt, Paula Rodrigues de Almeida, Daniela Müller de Quevedo, Horst-Günter Rubahn, Fernando Rosado Spilki
Source: Chemosensors, Vol 9, Iss 2, p 19 (2021)
Publisher Information: MDPI AG, 2021.
Publication Year: 2021
Collection: LCC:Biochemistry
Subject Terms: immunomagnetic separation, IMS-qPCR, ultracentrifugation, HAdV, scanning electron microscopy, helium ion microscopy, Biochemistry, QD415-436
More Details: The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2227-9040
Relation: https://www.mdpi.com/2227-9040/9/2/19; https://doaj.org/toc/2227-9040
DOI: 10.3390/chemosensors9020019
Access URL: https://doaj.org/article/9a6f200031584766a5c51dbaa095c121
Accession Number: edsdoj.9a6f200031584766a5c51dbaa095c121
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=22279040&ISBN=&volume=9&issue=2&date=20210101&spage=19&pages=19-19&title=Chemosensors&atitle=Functionalized%20Surfaces%20as%20a%20Tool%20for%20Virus%20Sensing%3A%20A%20Demonstration%20of%20Human%20mastadenovirus%20Detection%20in%20Environmental%20Waters&aulast=Juliana%20Schons%20Gularte&id=DOI:10.3390/chemosensors9020019
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/9a6f200031584766a5c51dbaa095c121
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.9a6f200031584766a5c51dbaa095c121
RelevancyScore: 894
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 893.9462890625
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Juliana+Schons+Gularte%22">Juliana Schons Gularte</searchLink><br /><searchLink fieldCode="AR" term="%22Roana+de+Oliveira+Hansen%22">Roana de Oliveira Hansen</searchLink><br /><searchLink fieldCode="AR" term="%22Meriane+Demoliner%22">Meriane Demoliner</searchLink><br /><searchLink fieldCode="AR" term="%22Jacek+Fiutowski%22">Jacek Fiutowski</searchLink><br /><searchLink fieldCode="AR" term="%22Ana+Karolina+Antunes+Eisen%22">Ana Karolina Antunes Eisen</searchLink><br /><searchLink fieldCode="AR" term="%22Fagner+Henrique+Heldt%22">Fagner Henrique Heldt</searchLink><br /><searchLink fieldCode="AR" term="%22Paula+Rodrigues+de+Almeida%22">Paula Rodrigues de Almeida</searchLink><br /><searchLink fieldCode="AR" term="%22Daniela+Müller+de+Quevedo%22">Daniela Müller de Quevedo</searchLink><br /><searchLink fieldCode="AR" term="%22Horst-Günter+Rubahn%22">Horst-Günter Rubahn</searchLink><br /><searchLink fieldCode="AR" term="%22Fernando+Rosado+Spilki%22">Fernando Rosado Spilki</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Chemosensors, Vol 9, Iss 2, p 19 (2021)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: MDPI AG, 2021.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2021
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Biochemistry
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22immunomagnetic+separation%22">immunomagnetic separation</searchLink><br /><searchLink fieldCode="DE" term="%22IMS-qPCR%22">IMS-qPCR</searchLink><br /><searchLink fieldCode="DE" term="%22ultracentrifugation%22">ultracentrifugation</searchLink><br /><searchLink fieldCode="DE" term="%22HAdV%22">HAdV</searchLink><br /><searchLink fieldCode="DE" term="%22scanning+electron+microscopy%22">scanning electron microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22helium+ion+microscopy%22">helium ion microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22QD415-436%22">QD415-436</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 2227-9040
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://www.mdpi.com/2227-9040/9/2/19; https://doaj.org/toc/2227-9040
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3390/chemosensors9020019
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/9a6f200031584766a5c51dbaa095c121" linkWindow="_blank">https://doaj.org/article/9a6f200031584766a5c51dbaa095c121</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.9a6f200031584766a5c51dbaa095c121
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.9a6f200031584766a5c51dbaa095c121
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3390/chemosensors9020019
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: 19
    Subjects:
      – SubjectFull: immunomagnetic separation
        Type: general
      – SubjectFull: IMS-qPCR
        Type: general
      – SubjectFull: ultracentrifugation
        Type: general
      – SubjectFull: HAdV
        Type: general
      – SubjectFull: scanning electron microscopy
        Type: general
      – SubjectFull: helium ion microscopy
        Type: general
      – SubjectFull: Biochemistry
        Type: general
      – SubjectFull: QD415-436
        Type: general
    Titles:
      – TitleFull: Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Juliana Schons Gularte
      – PersonEntity:
          Name:
            NameFull: Roana de Oliveira Hansen
      – PersonEntity:
          Name:
            NameFull: Meriane Demoliner
      – PersonEntity:
          Name:
            NameFull: Jacek Fiutowski
      – PersonEntity:
          Name:
            NameFull: Ana Karolina Antunes Eisen
      – PersonEntity:
          Name:
            NameFull: Fagner Henrique Heldt
      – PersonEntity:
          Name:
            NameFull: Paula Rodrigues de Almeida
      – PersonEntity:
          Name:
            NameFull: Daniela Müller de Quevedo
      – PersonEntity:
          Name:
            NameFull: Horst-Günter Rubahn
      – PersonEntity:
          Name:
            NameFull: Fernando Rosado Spilki
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Type: published
              Y: 2021
          Identifiers:
            – Type: issn-print
              Value: 22279040
          Numbering:
            – Type: volume
              Value: 9
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: Chemosensors
              Type: main
ResultId 1