Bibliographic Details
| Title: |
Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters |
| Authors: |
Juliana Schons Gularte, Roana de Oliveira Hansen, Meriane Demoliner, Jacek Fiutowski, Ana Karolina Antunes Eisen, Fagner Henrique Heldt, Paula Rodrigues de Almeida, Daniela Müller de Quevedo, Horst-Günter Rubahn, Fernando Rosado Spilki |
| Source: |
Chemosensors, Vol 9, Iss 2, p 19 (2021) |
| Publisher Information: |
MDPI AG, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Biochemistry |
| Subject Terms: |
immunomagnetic separation, IMS-qPCR, ultracentrifugation, HAdV, scanning electron microscopy, helium ion microscopy, Biochemistry, QD415-436 |
| More Details: |
The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2227-9040 |
| Relation: |
https://www.mdpi.com/2227-9040/9/2/19; https://doaj.org/toc/2227-9040 |
| DOI: |
10.3390/chemosensors9020019 |
| Access URL: |
https://doaj.org/article/9a6f200031584766a5c51dbaa095c121 |
| Accession Number: |
edsdoj.9a6f200031584766a5c51dbaa095c121 |
| Database: |
Directory of Open Access Journals |
