Bibliographic Details
| Title: |
Discrimination of Anti-Donor Response in Allogeneic Transplantation Using an Alloreactive T-Cell Detection Assay |
| Authors: |
Ryosuke Arata, Naoki Tanimine, Akhmet Seidakhmetov, Kentaro Ide, Yuka Tanaka, Hideki Ohdan |
| Source: |
Transplant International, Vol 38 (2025) |
| Publisher Information: |
Frontiers Media S.A., 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Specialties of internal medicine |
| Subject Terms: |
immune monitoring, allogeneic transplantation, alloreactive T-cell, rejection, tolerance, Specialties of internal medicine, RC581-951 |
| More Details: |
Understanding donor-reactive T-cell behavior post-transplantation is challenging owing to the rarity and diversity of these cells. Here, we aimed to evaluate the relevance of an assay for rapidly detecting alloreactive T cells in a mouse transplantation model. After 18 h of one-way mixed lymphocyte reaction (MLR) culture with pre-activated donor-derived stimulators, CD4+ and CD8+ donor-reactive T cells were identified by CD154 and CD137 expression, respectively. Using full MHC mismatched mouse skin transplant models, we observed an increased donor-reactive T-cell proportion by direct presentation with elevated interferon gamma and granzyme B production 7 days post-transplantation, before graft rejection. Immunosuppression with CTLA-4 IgG and anti-CD154 antibody varied depending on donor-recipient strain combinations. On day 7, donor-reactive CD8+ T-cell proportions were lower in the tolerance model (BALB/c to C3H/HeJ) than in the rejection model (BALB/c to C57BL/6); conventional proliferation readout after 4 days of MLR could not distinguish these responses. Overall, although the conventional readout for evaluating T-cell proliferation following an MLR quantifies the precursor frequency of alloreactive T cells, the assay reported herein assesses T-cell activation markers after a short-term MLR to characterize immediate immune status. These findings offer a promising tool to elucidate immune responses post-transplantation. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1432-2277 |
| Relation: |
https://www.frontierspartnerships.org/articles/10.3389/ti.2025.13879/full; https://doaj.org/toc/1432-2277 |
| DOI: |
10.3389/ti.2025.13879 |
| Access URL: |
https://doaj.org/article/9a5107ac4a9d4a04aac857695bf3c412 |
| Accession Number: |
edsdoj.9a5107ac4a9d4a04aac857695bf3c412 |
| Database: |
Directory of Open Access Journals |
