Bibliographic Details
| Title: |
Escherichia coli O157:H7 tir 255 T > A allele strains differ in chromosomal and plasmid composition |
| Authors: |
Margaret D. Weinroth, Michael L. Clawson, Gregory P. Harhay, Mark Eppinger, Dayna M. Harhay, Timothy P. L. Smith, James L. Bono |
| Source: |
Frontiers in Microbiology, Vol 14 (2023) |
| Publisher Information: |
Frontiers Media S.A., 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
whole genome sequencing (WGS), comparative genomics, bacterial genomics, STEC, Plasmids, Bacteriophage, Microbiology, QR1-502 |
| More Details: |
Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains with the T allele in the translocated intimin receptor polymorphism (tir) 255 A > T gene associate with human disease more than strains with an A allele; however, the allele is not thought to be the direct cause of this difference. We sequenced a diverse set of STEC O157:H7 strains (26% A allele, 74% T allele) to identify linked differences that might underlie disease association. The average chromosome and pO157 plasmid size and gene content were significantly greater within the tir 255 A allele strains. Eighteen coding sequences were unique to tir 255 A allele chromosomes, and three were unique to tir 255 T allele chromosomes. There also were non-pO157 plasmids that were unique to each tir 255 allele variant. The overall average number of prophages did not differ between tir 255 allele strains; however, there were different types between the strains. Genomic and mobile element variation linked to the tir 255 polymorphism may account for the increased frequency of the T allele isolates in human disease. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1303387/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2023.1303387 |
| Access URL: |
https://doaj.org/article/d9a0306cf8ed46d69c809ab1697f8851 |
| Accession Number: |
edsdoj.9a0306cf8ed46d69c809ab1697f8851 |
| Database: |
Directory of Open Access Journals |
