Bibliographic Details
| Title: |
Genistein attenuated gastric inflammation and apoptosis in Helicobacter pylori-induced gastropathy in rats |
| Authors: |
Prasong Siriviriyakul, Duangporn Werawatganon, Nisarat Phetnoo, Kanjana Somanawat, Tanittha Chatsuwan, Naruemon Klaikeaw, Maneerat Chayanupatkul |
| Source: |
BMC Gastroenterology, Vol 20, Iss 1, Pp 1-9 (2020) |
| Publisher Information: |
BMC, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Diseases of the digestive system. Gastroenterology |
| Subject Terms: |
Genistein, H. pylori, Apoptosis, Gastric inflammation, Gastropathy, Diseases of the digestive system. Gastroenterology, RC799-869 |
| More Details: |
Abstract Background Helicobacter pylori (H. pylori) infection is a major cause of chronic gastritis, peptic ulcer diseases and cancer. Genistein (4′,5,7-trihydroxyisoflavone), a tyrosine-specific-protein kinase inhibitor, has been shown to exert an anti-inflammatory property. The aim of this study was to examine the treatment effects of genistein and its mechanisms in rats with H. pylori infection. Methods Eighteen male Sprague-Dawley rats were divided into three groups (6 rats per group): (1) control group (Con); (2) H. pylori infected group (HP): the rats were inoculated with H. pylori (108− 1010 CFU/mL; 1 mL/rat.) for 3 consecutive days; and (3) HP + genistein group (HP + Gen): the rats were inoculated with H. pylori as above. Then, they were gavaged with genistein (16 mg/kg BW) for 14 days. Gastric tissue was used for the determination of nuclear factor (NF)-κB expression by immunohistochemistry (IHC), degree of apoptosis by the terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL) reaction, and histopathology. Serum samples were used to measure the levels of tumor necrosis factor-alpha (TNF-α) and cytokine-induced neutrophil chemoattractant-1 (CINC-1). Results Rats in the HP group had significantly higher levels of pro-inflammatory mediators, NF-κB expression and apoptotic cells when compared with the Con group, and these markers significantly decreased in HP + Gen group when compared with the HP group. The histopathology of HP group showed moderate gastric inflammation and many HP colonization. Gastric pathology in HP + Gen group demonstrated the attenuation of inflammatory cell infiltration and H. pylori colonization. Conclusion Genistein exerted its gastroprotective effects through the reduction of pro-inflammatory mediators, nuclear receptor NF-κB expression and gastric mucosal apoptosis in rats with H. pylori-induced gastropathy. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-230X |
| Relation: |
https://doaj.org/toc/1471-230X |
| DOI: |
10.1186/s12876-020-01555-x |
| Access URL: |
https://doaj.org/article/9992d7b5e3bc437cbddd511bef0bb0d9 |
| Accession Number: |
edsdoj.9992d7b5e3bc437cbddd511bef0bb0d9 |
| Database: |
Directory of Open Access Journals |
