Bibliographic Details
| Title: |
A dormant cancer mouse model established by combining preimmune strategy with mVenus-p27K- system |
| Authors: |
Musitaba MUTAILIFU, WANG Junjie, QIAN Yunzhen, CHEN Suyuan, SHAO Da, ZHANG Zhigang, LI Dongxue |
| Source: |
Shanghai Jiaotong Daxue xuebao. Yixue ban, Vol 44, Iss 9, Pp 1104-1114 (2024) |
| Publisher Information: |
Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science), 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
pancreatic ductal adenocarcinoma (pdac), dormant cancer cell, preimmunized mouse, mvenus-p27k- system, animal model, Medicine |
| More Details: |
Objective·To establish a mouse model with dormant cancer and no obvious metastasis by combining the preimmune strategy with the mVenus-p27K－ cell G0 phase indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system.Methods·The KPC1199 mouse pancreatic cancer cell line was transfected with the mVenus-p27K－ cell G0 phase indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system to construct a stable expression cell line, KPC1199-PDL. After being cultured in the serum-free condition, KPC1199-PDL cells were sorted into mVenus (+) cells and mVenus (-) cells by flow cytometry, and the expression of G0 phase-related genes was verified by real-time fluorescence quantitative PCR (qPCR). Sensitivity of KPC1199-PDL cells to diphtheria toxin (DTX) and ganciclovir (GCV) was evaluated by CCK-8 assay. A transsplenic portal vein-hepatic metastasis model was constructed in wild-type C57BL/6 mice to validate the function of KPC1199-PDL cells in vivo by immunofluorescence technology. The KPC1199-PDL cells were injected subcutaneously into C57BL/6 mice, followed by in situ injection of DTX and GCV to ablate subcutaneous tumors 5 d later, to obtain preimmunized mice. The transsplenic portal vein-hepatic metastasis models were constructed in these mice. Bioluminescence imaging was used to evaluate subcutaneous tumor ablation and hepatic metastasis in the mice, and immunofluorescence assay was used to detect the distribution and dormant state of tumor cells in the livers of preimmunize mice.Results·The three tool systems were stably expressed in KPC1199-PDL cells, and their proliferative ability was not affected. In the serum starving condition, some KPC1199-PDL cells expressed the mVenus protein, indicating entry into the G0 phase; the mVenus (+) cells sorted out by flow cytometry exhibited significantly higher expression of G0 phase-related genes (all P |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
Chinese |
| ISSN: |
1674-8115 |
| Relation: |
https://xuebao.shsmu.edu.cn/article/2024/1674-8115/1674-8115-2024-44-9-1104.shtml; https://doaj.org/toc/1674-8115 |
| DOI: |
10.3969/j.issn.1674-8115.2024.09.005 |
| Access URL: |
https://doaj.org/article/d99722cb31a74969a0af800a8e9c1fbb |
| Accession Number: |
edsdoj.99722cb31a74969a0af800a8e9c1fbb |
| Database: |
Directory of Open Access Journals |
