Academic Journal
Gene expression profiling of nasal inflammation induced by diesel particles using an in vivo system
| Title: | Gene expression profiling of nasal inflammation induced by diesel particles using an in vivo system |
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| Authors: | Bongkyun Park, Musun Park, Kyuhyung Jo, Chan-Sik Kim, Su-Jin Baek |
| Source: | Ecotoxicology and Environmental Safety, Vol 252, Iss , Pp 114586- (2023) |
| Publisher Information: | Elsevier, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Environmental pollution LCC:Environmental sciences |
| Subject Terms: | Diesel particle, Gene expression signature, Biomarker, Nasal inflammation, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| More Details: | Korean diesel particulate matter 20 (KDP20) is a pollutant comprising a complex mixture of carbon and chemical irritants. Although particulate matter and nasal inflammation are strongly associated, the underlying molecular mechanism based on systematic transcriptome analysis remains unknown. In this study, genome-wide gene expression profiles of mouse nasal tissues were determined following exposure to KDP20 for 5 and 10 days and compared with those of the control (n = 4/group). We identified 758 significant differentially expressed genes (DEGs) and classified them as 5-day-specific, 10-day-specific, and common among groups based on their expression patterns. The terms “regulation of alpha-beta T cell differentiation,” “macrophage differentiation,” and “cell adhesion mediated by integrin” were significantly enriched in each group. Receiver operating characteristic analysis revealed six genes as potential predictive biomarkers. The differential expression of these six genes was validated using quantitative RT-PCR (n = 3/group). Furthermore, a possible mechanism for nasal inflammation was suggested through the binding analysis between metal ions and genes. The genes identified in this study may play important roles in regulating the mechanism of nasal inflammation induced by diesel particles, especially immune cell regulation, and may function as markers for diesel particle-induced nasal inflammation. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0147-6513 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651323000908; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2023.114586 |
| Access URL: | https://doaj.org/article/97f92bd95a2a49539754b8570407cef9 |
| Accession Number: | edsdoj.97f92bd95a2a49539754b8570407cef9 |
| Database: | Directory of Open Access Journals |
| ISSN: | 01476513 |
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| DOI: | 10.1016/j.ecoenv.2023.114586 |
| Published in: | Ecotoxicology and Environmental Safety |
| Language: | English |