Bibliographic Details
| Title: |
Differential RNA editing landscapes in host cell versus the SARS-CoV-2 genome |
| Authors: |
Małgorzata Kurkowiak, Sarah Fletcher, Alison Daniels, Paweł Mozolewski, Domenico Alessandro Silvestris, Ewelina Król, Natalia Marek-Trzonkowska, Ted Hupp, Christine Tait-Burkard |
| Source: |
iScience, Vol 26, Iss 11, Pp 108031- (2023) |
| Publisher Information: |
Elsevier, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Science |
| Subject Terms: |
Virology, Evolutionary biology, Science |
| More Details: |
Summary: The SARS-CoV-2 pandemic was defined by the emergence of new variants formed through virus mutation originating from random errors not corrected by viral proofreading and/or the host antiviral response introducing mutations into the viral genome. While sequencing information hints at cellular RNA editing pathways playing a role in viral evolution, here, we use an in vitro human cell infection model to assess RNA mutation types in two SARS-CoV-2 strains representing the original and the alpha variants. The variants showed both different cellular responses and mutation patterns with alpha showing higher mutation frequency with most substitutions observed being C-U, indicating an important role for apolipoprotein B mRNA editing catalytic polypeptide-like editing. Knockdown of select APOBEC3s through RNAi increased virus production in the original virus, but not in alpha. Overall, these data suggest a deaminase-independent anti-viral function of APOBECs in SARS-CoV-2 while the C-U editing itself might function to enhance genetic diversity enabling evolutionary adaptation. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004223021089; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2023.108031 |
| Access URL: |
https://doaj.org/article/96e6a4a0653448ff93e4d1d0e6bd2996 |
| Accession Number: |
edsdoj.96e6a4a0653448ff93e4d1d0e6bd2996 |
| Database: |
Directory of Open Access Journals |
