Crebanine N-oxide, a natural aporphine alkaloid isolated from Stephania hainanensis, induces apoptosis and autophagy in human gastric cancer SGC-7901 cells
| Title: | Crebanine N-oxide, a natural aporphine alkaloid isolated from Stephania hainanensis, induces apoptosis and autophagy in human gastric cancer SGC-7901 cells |
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| Authors: | Zheng-Wen Wang, Hao Liu, Geng-Tai Ye, Zhi-Yong Sheng, Yan-Feng Hu, Yin-Feng Tan, Guo-Xin Li |
| Source: | Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 5, Pp 224-231 (2020) |
| Publisher Information: | Wolters Kluwer Medknow Publications, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Arctic medicine. Tropical medicine LCC:Biology (General) |
| Subject Terms: | crebanine n-oxide, gastric cancer, sgc-7901 cells, apoptosis, autophagy, Arctic medicine. Tropical medicine, RC955-962, Biology (General), QH301-705.5 |
| More Details: | Objective: To investigate the cytotoxic effects and the potential mechanisms of crebanine N-oxide in SGC-7901 gastric adenocarcinoma cells. Methods: The cytotoxicity of crebanine N-oxide was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay and cellular morphology was observed under a microscope. Cell apoptosis was determined by flow cytometry using propidium iodide staining. The expression levels of apoptotic-related proteins, cleaved caspase-3, cytochrome C, p53 and Bax, and autophagy- related proteins p62, beclin1 and LC3 were detected by Western blotting assays. Results: Crebanine N-oxide treatment significantly inhibited the proliferation of SGC-7901 cells in a dose-dependent and time- dependent manner via induction of G2-phase cell cycle arrest, apoptosis, and autophagy in SGC-7901 cells. Conclusions: Crebanine N-oxide could inhibit the growth of gastric cancer cells by promoting apoptosis and autophagy and could be used as a potential agent for treating gastric cancer. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2221-1691 2588-9222 |
| Relation: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=5;spage=224;epage=231;aulast=Wang; https://doaj.org/toc/2221-1691; https://doaj.org/toc/2588-9222 |
| DOI: | 10.4103/2221-1691.281466 |
| Access URL: | https://doaj.org/article/95e7911a4cea4ce088787bf9b493b02f |
| Accession Number: | edsdoj.95e7911a4cea4ce088787bf9b493b02f |
| Database: | Directory of Open Access Journals |
| ISSN: | 22211691 25889222 |
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| DOI: | 10.4103/2221-1691.281466 |
| Published in: | Asian Pacific Journal of Tropical Biomedicine |
| Language: | English |