Optical trapping in vivo: theory, practice, and applications
| Title: | Optical trapping in vivo: theory, practice, and applications |
|---|---|
| Authors: | Favre-Bulle Itia A., Stilgoe Alexander B., Scott Ethan K., Rubinsztein-Dunlop Halina |
| Source: | Nanophotonics, Vol 8, Iss 6, Pp 1023-1040 (2019) |
| Publisher Information: | De Gruyter, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Physics |
| Subject Terms: | optical tweezers, scattering, microscopy, imaging, structured light, complex biological systems, Physics, QC1-999 |
| More Details: | Since the time of their introduction, optical tweezers (OTs) have grown to be a powerful tool in the hands of biologists. OTs use highly focused laser light to guide, manipulate, or sort target objects, typically in the nanoscale to microscale range. OTs have been particularly useful in making quantitative measurements of forces acting in cellular systems; they can reach inside living cells and be used to study the mechanical properties of the fluids and structures that they contain. As all the measurements are conducted without physically contacting the system under study, they also avoid complications related to contamination and tissue damage. From the manipulation of fluorescent nanodiamonds to chromosomes, cells, and free-swimming bacteria, OTs have now been extended to challenging biological systems such as the vestibular system in zebrafish. Here, we will give an overview of OTs, the complications that arise in carrying out OTs in vivo, and specific OT methods that have been used to address a range of otherwise inaccessible biological questions. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2192-8606 2192-8614 |
| Relation: | https://doaj.org/toc/2192-8606; https://doaj.org/toc/2192-8614 |
| DOI: | 10.1515/nanoph-2019-0055 |
| Access URL: | https://doaj.org/article/9571350089934f29b8d2aa51f9493dab |
| Accession Number: | edsdoj.9571350089934f29b8d2aa51f9493dab |
| Database: | Directory of Open Access Journals |
| ISSN: | 21928606 21928614 |
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| DOI: | 10.1515/nanoph-2019-0055 |
| Published in: | Nanophotonics |
| Language: | English |