Academic Journal
Role of P2 X7 receptor during focused ultrasound induced blood brain barrier modulation
| Title: | Role of P2 X7 receptor during focused ultrasound induced blood brain barrier modulation |
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| Authors: | Junwon Park, Young Cheol Na, Jihyeon Lee, Younghee Seo, Hojin Kim, Sangheon Han, Byeong-Wook Song, Won Seok Chang |
| Source: | Scientific Reports, Vol 15, Iss 1, Pp 1-15 (2025) |
| Publisher Information: | Nature Portfolio, 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Abstract Although low-intensity focused ultrasound (LiFUS) with microbubbles is used to temporally open the blood-brain barrier (BBB), the underlying mechanism is not fully understood. This study aimed to analyze BBB-related alterations in the brain microenvironment after LiFUS, with a focus on the involvement of the purinergic P2 X7 receptor. Sprague-Dawley rats were sonicated with LiFUS at 0.3 MPa energy. The impact of LiFUS on the P2 X7 receptor and inflammatory-related proteins, including NLRP3 and interleukin-1β, was analyzed through western blotting. The BBB-associated tight junction proteins, zonula occludens-1 (ZO-1) and occludin, were also analyzed. BBB permeability was assessed by quantifying the amount of Evans blue dye penetration using spectrophotometry. Furthermore, the safety of the sonication procedure was verified via terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and hematoxylin and eosin staining. Substantial increases in the P2 X7 receptor and its downstream signaling were confirmed after sonicating the BBB with LiFUS for 1 h (p |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-83913-3 |
| Access URL: | https://doaj.org/article/94851c3cb4c24c5b8bef0d7d0d38474d |
| Accession Number: | edsdoj.94851c3cb4c24c5b8bef0d7d0d38474d |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
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| DOI: | 10.1038/s41598-024-83913-3 |
| Published in: | Scientific Reports |
| Language: | English |