Academic Journal
Discovery of key genes as novel biomarkers specifically associated with HPV-negative cervical cancer
| Title: | Discovery of key genes as novel biomarkers specifically associated with HPV-negative cervical cancer |
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| Authors: | Yi Liu, Yichi Xu, Wenxiao Jiang, Huihui Ji, Zhi-wei Wang, Xueqiong Zhu |
| Source: | Molecular Therapy: Methods & Clinical Development, Vol 21, Iss , Pp 492-506 (2021) |
| Publisher Information: | Elsevier, 2021. |
| Publication Year: | 2021 |
| Collection: | LCC:Genetics LCC:Cytology |
| Subject Terms: | HPV-negative cervical cancer, HPV-positive cervical cancer, MEX3A, TTYH3, prognosis, ETV4, Genetics, QH426-470, Cytology, QH573-671 |
| More Details: | Cervical cancer is a common female malignancy that is mainly caused by human papillomavirus (HPV) infection. However, the incidence of HPV-negative cervical cancer has shown an increasing trend in recent years. Because the mechanism of HPV-negative cervical cancer development is unclear, this study aims to find the pattern of differential gene expression in HPV-negative cervical cancer and verify the underlying potential mechanism. Differentially expressed genes were compared among HPV-positive cervical cancer, HPV-negative cervical cancer, and normal cervical tissues retrieved from TCGA. Subsequently, dysregulated differentially expressed genes specifically existed in HPV-negative cervical cancer tissues and HPV-negative cell lines were validated by qRT-PCR, western blotting, and immunohistochemical staining. We found seventeen highly expressed genes that were particularly associated with HPV-negative cervical cancer from analysis of TCGA database. Among the 17 novel genes, 7 genes (preferentially expressed antigen in melanoma [PRAME], HMGA2, ETS variant 4 [ETV4], MEX3A, TM7SF2, SLC19A1, and tweety-homologs 3 [TTYH3]) displayed significantly elevated expression in HPV-negative cervical cancer cells and HPV-negative cervical cancer tissues. Additionally, higher expression of MEX3A and TTYH3 was associated with a shorter overall survival of patients with HPV-negative cervical cancer. Our study implies that these seven genes are more likely to provide novel insights into the occurrence and progression of HPV-negative cervical cancer. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2329-0501 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S232905012100067X; https://doaj.org/toc/2329-0501 |
| DOI: | 10.1016/j.omtm.2021.03.026 |
| Access URL: | https://doaj.org/article/938945cea20d4e95995df9c28b890097 |
| Accession Number: | edsdoj.938945cea20d4e95995df9c28b890097 |
| Database: | Directory of Open Access Journals |
| ISSN: | 23290501 |
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| DOI: | 10.1016/j.omtm.2021.03.026 |
| Published in: | Molecular Therapy: Methods & Clinical Development |
| Language: | English |