Academic Journal
M2 macrophages‐derived exosomal microRNA-501-3p promotes the progression of lung cancer via targeting WD repeat domain 82
| Title: | M2 macrophages‐derived exosomal microRNA-501-3p promotes the progression of lung cancer via targeting WD repeat domain 82 |
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| Authors: | Jie Lei, Peng Chen, Feng Zhang, Na Zhang, Jianfei Zhu, Xiaoping Wang, Tao Jiang |
| Source: | Cancer Cell International, Vol 21, Iss 1, Pp 1-13 (2021) |
| Publisher Information: | BMC, 2021. |
| Publication Year: | 2021 |
| Collection: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LCC:Cytology |
| Subject Terms: | Lung cancer, M2 macrophages-derived exosome, MicroRNA-501-3p, WD repeat domain 82, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671 |
| More Details: | Abstract Background Exosomes are known to transmit microRNAs (miRNAs) to affect cancer progression, while the role of M2 macrophages-derived exosomes (M2 exosomes) conveying miR-501-3p in lung cancer (LC) remains unknown. We aim to explore the role of exosomal miR-501-3p in LC development via targeting WD repeat domain 82 (WDR82). Methods Lung cancer tissue and normal tissue specimens were collected, in which tumor-associated macrophages (TAM) were measured by immunohistochemistry. M2 macrophages were induced and treated with altered miR-501-3p, and then the exosomes were extracted and identified. MiR-501-3p and WDR82 expression in LC tissues and cell liens was determined. The predictive role of miR-501-3p in prognosis of LC patients was assessed, and the proliferation, colony formation ability, invasion, migration and apoptosis of the LC cells were determined. Targeting relationship between miR-501-3p and WDR82 was confirmed. Results TAM level was elevated in lung cancer tissues. MiR-501-3p was upregulated while WDR82 was downregulated in LC tissues and cell lines, and the M2 exosomes further upregulated miR-501-3p. M2 exosomes and exosomal miR-501-3p promoted LC cell growth. MiR-501-3p inhibition reversed the effect of M2 exosomes on LC cells. WDR82 was confirmed as a target gene of miR-501-3p. Conclusion M2 macrophages-derived exosomal miR-501-3p promotes the progression of LC via downregulating WDR82. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1475-2867 |
| Relation: | https://doaj.org/toc/1475-2867 |
| DOI: | 10.1186/s12935-021-01783-5 |
| Access URL: | https://doaj.org/article/9203b62be7f243f98f68eb8581bfa430 |
| Accession Number: | edsdoj.9203b62be7f243f98f68eb8581bfa430 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 14752867 |
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| DOI: | 10.1186/s12935-021-01783-5 |
| Published in: | Cancer Cell International |
| Language: | English |