Academic Journal
eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization.
| Title: | eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization. |
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| Authors: | Claudia Matthaeus, Xiaoming Lian, Séverine Kunz, Martin Lehmann, Cheng Zhong, Carola Bernert, Ines Lahmann, Dominik N Müller, Maik Gollasch, Oliver Daumke |
| Source: | PLoS ONE, Vol 14, Iss 10, p e0223620 (2019) |
| Publisher Information: | Public Library of Science (PLoS), 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Endothelial nitric oxide synthase (eNOS)-related vessel relaxation is a highly coordinated process that regulates blood flow and pressure and is dependent on caveolae. Here, we investigated the role of caveolar plasma membrane stabilization by the dynamin-related ATPase EHD2 on eNOS-nitric oxide (NO)-dependent vessel relaxation. Loss of EHD2 in small arteries led to increased numbers of caveolae that were detached from the plasma membrane. Concomitantly, impaired relaxation of mesenteric arteries and reduced running wheel activity were observed in EHD2 knockout mice. EHD2 deletion or knockdown led to decreased production of nitric oxide (NO) although eNOS expression levels were not changed. Super-resolution imaging revealed that eNOS was redistributed from the plasma membrane to internalized detached caveolae in EHD2-lacking tissue or cells. Following an ATP stimulus, reduced cytosolic Ca2+ peaks were recorded in human umbilical vein endothelial cells (HUVECs) lacking EHD2. Our data suggest that EHD2-controlled caveolar dynamics orchestrates the activity and regulation of eNOS/NO and Ca2+ channel localization at the plasma membrane. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1932-6203 |
| Relation: | https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0223620 |
| Access URL: | https://doaj.org/article/90f0a162800541d2975c2545f206a29d |
| Accession Number: | edsdoj.90f0a162800541d2975c2545f206a29d |
| Database: | Directory of Open Access Journals |
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| ISSN: | 19326203 |
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| DOI: | 10.1371/journal.pone.0223620 |
| Published in: | PLoS ONE |
| Language: | English |