Bibliographic Details
| Title: |
Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen |
| Authors: |
Shigeo Toh, Naoki Takata, Eigo Ando, Yosuke Toda, Yin Wang, Yuki Hayashi, Nobutaka Mitsuda, Soichiro Nagano, Toru Taniguchi, Toshinori Kinoshita |
| Source: |
Frontiers in Plant Science, Vol 12 (2021) |
| Publisher Information: |
Frontiers Media S.A., 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Plant culture |
| Subject Terms: |
PUMP, PM H+-ATPase, guard cell, stomatal conductance, hybrid aspen, Plant culture, SB1-1110 |
| More Details: |
Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-462X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fpls.2021.766037/full; https://doaj.org/toc/1664-462X |
| DOI: |
10.3389/fpls.2021.766037 |
| Access URL: |
https://doaj.org/article/9096d1cecd5c452b81701b0c929622ff |
| Accession Number: |
edsdoj.9096d1cecd5c452b81701b0c929622ff |
| Database: |
Directory of Open Access Journals |
