Bibliographic Details
| Title: |
Single-cell transcriptomic analysis reveals a decrease in the frequency of macrophage-RGS1high subsets in patients with osteoarticular tuberculosis |
| Authors: |
Ying Jiang, Xinqiang Zhang, Bo Wang, Liping Tang, Xin Liu, Xiudong Ding, Yueming Dong, Hong Lei, Di Wang, Huicheng Feng |
| Source: |
Molecular Medicine, Vol 30, Iss 1, Pp 1-14 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Therapeutics. Pharmacology
LCC:Biochemistry |
| Subject Terms: |
Osteoarticular tuberculosis, scRNA, Biomarker, Macrophages, Ferroptosis, Therapeutics. Pharmacology, RM1-950, Biochemistry, QD415-436 |
| More Details: |
Abstract Background Cell subsets differentially modulate host immune responses to Mycobacterium tuberculosis (MTB) infection. However, the nature and functions of these subsets against osteoarticular tuberculosis (OTB) are unclear. Here, we aimed to understand the phenotypes and functions of immune cell subsets in patients with OTB using single-cell RNA sequencing (scRNA-Seq). Methods Pathological and healthy adjacent tissues were isolated from patients with OTB and subjected to scRNA-Seq. Unsupervised clustering of cells was performed based on gene expression profiles, and uniform manifold approximation and projection was used for clustering visualization. Results Thirteen cell subsets were identified in OTB tissues. scRNA-seq datasets of patients and healthy controls (HCs) showed that infection changed the frequency of immune cell subsets in OTB tissues. Myeloid cell examination revealed nine subsets. The frequency of macrophage-RGS1high subsets decreased in OTB tissues; this increased MTB susceptibility in an SLC7A11/ferroptosis-dependent manner. Immunohistochemistry assays and flow cytometry for patients with OTB and osteoarticular bacterial infection (OBI) and HCs verified that the frequency of macrophage-RGS1high subset decreased in OTB tissues and blood samples, thereby distinguishing patients with OTB from HCs and patients with OBI. Conclusion The macrophage-RGS1high subset levels were decreased in patients with OTB, and would be up-regulated after effective treatment. Therefore, the clinical significance of this study is to discover that macrophage-RGS1high subset may serve as a potential biomarker for OTB diagnosis and treatment efficacy monitoring. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1528-3658 |
| Relation: |
https://doaj.org/toc/1528-3658 |
| DOI: |
10.1186/s10020-024-00886-9 |
| Access URL: |
https://doaj.org/article/a8ff9f2ef337405f90ab3d1817fdc224 |
| Accession Number: |
edsdoj.8ff9f2ef337405f90ab3d1817fdc224 |
| Database: |
Directory of Open Access Journals |
