| Title: |
MicroRNA profile of circulating CD4+ T cells in aged patients with atherosclerosis obliterans |
| Authors: |
Siwen Wang, Suiting Jiang, Ruijia Feng, Jiawei Liu, Longshan Liu, Jin Cui, Yi Shi, Junjie Ning, Benyuan Jia, Zuojun Hu, Shenming Wang |
| Source: |
BMC Cardiovascular Disorders, Vol 22, Iss 1, Pp 1-13 (2022) |
| Publisher Information: |
BMC, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Diseases of the circulatory (Cardiovascular) system |
| Subject Terms: |
Atherosclerosis, Atherosclerosis obliterans, Circulating CD4+ T cells, MicroRNAs, Molecular signature, Diseases of the circulatory (Cardiovascular) system, RC666-701 |
| More Details: |
Abstract Background To evaluate the specificity of the expression patterns of microRNAs (miRNAs) in circulating CD4+ T cells in aged patients with atherosclerosis obliterans (ASO). Methods A comprehensive miRNA expression study was conducted using a miRNA microarray of CD4+ T cells isolated from peripheral blood mononuclear cells (PBMCs) of 33 patients with ASO and 24 healthy donors. A t test was used for statistical analysis, and the average linkage method was used for hierarchical clustering. The results were validated by qRT–PCR. Putative targeted pathways associated with validated miRNAs were predicted with the online software DIANA miRPath. Results We identified 44 miRNAs based on a cutoff value of a 1.3-fold change in expression between the two groups, with 18 miRNAs showing a false discovery rate (FDR) p value |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-2261 |
| Relation: |
https://doaj.org/toc/1471-2261 |
| DOI: |
10.1186/s12872-022-02616-7 |
| Access URL: |
https://doaj.org/article/c8eea729d1b8472eb12843d9a2b4be0e |
| Accession Number: |
edsdoj.8eea729d1b8472eb12843d9a2b4be0e |
| Database: |
Directory of Open Access Journals |
