Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning
| Title: | Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning |
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| Authors: | Sophie M Morgani, Jakob J Metzger, Jennifer Nichols, Eric D Siggia, Anna-Katerina Hadjantonakis |
| Source: | eLife, Vol 7 (2018) |
| Publisher Information: | eLife Sciences Publications Ltd, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Medicine LCC:Science LCC:Biology (General) |
| Subject Terms: | mammalian Embryo, epiblast, gastrulation, pluripotent stem cells, micropatterns, Medicine, Science, Biology (General), QH301-705.5 |
| More Details: | During gastrulation epiblast cells exit pluripotency as they specify and spatially arrange the three germ layers of the embryo. Similarly, human pluripotent stem cells (PSCs) undergo spatially organized fate specification on micropatterned surfaces. Since in vivo validation is not possible for the human, we developed a mouse PSC micropattern system and, with direct comparisons to mouse embryos, reveal the robust specification of distinct regional identities. BMP, WNT, ACTIVIN and FGF directed mouse epiblast-like cells to undergo an epithelial-to-mesenchymal transition and radially pattern posterior mesoderm fates. Conversely, WNT, ACTIVIN and FGF patterned anterior identities, including definitive endoderm. By contrast, epiblast stem cells, a developmentally advanced state, only specified anterior identities, but without patterning. The mouse micropattern system offers a robust scalable method to generate regionalized cell types present in vivo, resolve how signals promote distinct identities and generate patterns, and compare mechanisms operating in vivo and in vitro and across species. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2050-084X |
| Relation: | https://elifesciences.org/articles/32839; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.32839 |
| Access URL: | https://doaj.org/article/8eea50c5e0f14b07bca739e2f1420f10 |
| Accession Number: | edsdoj.8eea50c5e0f14b07bca739e2f1420f10 |
| Database: | Directory of Open Access Journals |
| ISSN: | 2050084X |
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| DOI: | 10.7554/eLife.32839 |
| Published in: | eLife |
| Language: | English |