Bibliographic Details
| Title: |
Identification of sex determination locus and development of marker combination in Vitis based on genotyping by target sequencing |
| Authors: |
Bohan Yang, Wei Wu, Junli Lv, Jiangyu Li, Yanfen Xu, Ling Yin, Jiang Lu, Peining Fu |
| Source: |
Fruit Research, Vol 3, Iss 1, Pp 1-9 (2023) |
| Publisher Information: |
Maximum Academic Press, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Plant culture |
| Subject Terms: |
grapevine, gbts technology, sex determination locus, genetic breeding, marker-assisted selection, Plant culture, SB1-1110 |
| More Details: |
The grapevine is an important and economically valuable fruit crop, with flower sex being a key genetic trait that directly affects grapevine yield and quality. Despite its significance, there is a lack of studies on sex-linked molecular markers that can assist in grapevine breeding. In this study, we developed a grapevine single nucleotide polymorphism (SNP) marker array using a combination of genotyping by target sequencing (GBTS) and capture-in-solution technology and applied it to marker-assisted selection (MAS) of grapevine gender. The SNP array could detect a total of 20,597 core SNPs and 97,453 multiple SNPs (mSNPs), covering over 99% of the grapevine genome on each chromosome. A total of 131 progenies from a cross between Vitis vinifera 'Cabernet Sauvignon' and Vitis pseudoreticulata 'Huadong1058' that exhibited segregated sex phenotypes were sequenced using this array. Through locus mapping and a genome-wide association study (GWAS), a locus on chromosome 2 (54.74−58.80 cM) that explained 98.6% of the phenotypic variation was identified. To further utilize this locus, a sex prediction marker combination consisting of two SNPs was developed, which accurately predicted the sex of 34 natural grapevine varieties/accessions. This study demonstrates the application of GBTS in grapevine breeding and provides a reliable MAS marker set for early-stage sex selection. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2769-4615 |
| Relation: |
https://doaj.org/toc/2769-4615 |
| DOI: |
10.48130/FruRes-2023-0031 |
| Access URL: |
https://doaj.org/article/a8eabf88ebbb4bf0a0943f6ceead2ff7 |
| Accession Number: |
edsdoj.8eabf88ebbb4bf0a0943f6ceead2ff7 |
| Database: |
Directory of Open Access Journals |
