Bibliographic Details
| Title: |
Antibody Properties Associate with Clinical Phenotype in LGI1 Encephalitis |
| Authors: |
Susann Ludewig, Leonie Salzburger, Alexander Goihl, Jana Rohne, Frank Leypoldt, Daniel Bittner, Emrah Düzel, Burkhart Schraven, Dirk Reinhold, Martin Korte, Péter Körtvélyessy |
| Source: |
Cells, Vol 12, Iss 2, p 282 (2023) |
| Publisher Information: |
MDPI AG, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Cytology |
| Subject Terms: |
LGI1 encephalitis, autoimmune encephalitis, pathophysiology encephalitis, synaptic plasticity, epitope of anti-LGI1 antibodies, Cytology, QH573-671 |
| More Details: |
Autoimmune encephalitis (AE) associated with autoantibodies against leucine-rich glioma-inactivated protein-1 (LGI1) can present with faciobrachial dystonic seizures (FBDS) and/or limbic encephalitis (LE). The reasons for this heterogeneity in phenotypes are unclear. We performed autoantibody (abs) characterization per patient, two patients suffering from LE and two from FBDS, using isolated antibodies specified with single amino acid epitope mapping. Electrophysiological slice recordings were conducted alongside spine density measurements, postsynaptic Alpha-amino-3-hydoxy-5-methyl-4-isoaxole-proprionate-receptors (AMPA-R) and N-methyl-D-aspartate-receptors receptor (NMDA-R) cluster counting. These results were correlated with the symptoms of each patient. While LGI1 abs from LE patients mainly interacted with the Leucine-rich repeat section of LGI1, abs from both FBDS patients also recognized the Epitempin section as well. Six-hour incubation of mouse hippocampal slices with LE patients autoantibodies but not from the FBDS patients resulted in a significant decline in long-term potentiation (p = 0.0015) or short-term plasticity at CA3-CA1 neurons and in decreased hippocampal synaptic density. Cluster differentiation showed no decrease in postsynaptic AMPA-R and NMDA-R. LGI1 autoantibodies selected by phenotype show an almost distinct epitope pattern, elicit disparate functional effects on hippocampal neurons, and cause divergent effects on spine density. This data illuminates potential pathomechanisms for disease heterogeneity in LGI1 AE. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2073-4409 |
| Relation: |
https://www.mdpi.com/2073-4409/12/2/282; https://doaj.org/toc/2073-4409 |
| DOI: |
10.3390/cells12020282 |
| Access URL: |
https://doaj.org/article/8e9dd7d9509b40e5b769d3c44285734c |
| Accession Number: |
edsdoj.8e9dd7d9509b40e5b769d3c44285734c |
| Database: |
Directory of Open Access Journals |
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